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The role of KLF4 in the development of human primordial germ cell

ABSTRACT: The role of KLF4 in the development of human primordial germ cell

PROVIDER: PRJNA1133677 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29755808_1.fastq.gz	Fastqsanger.gz
	SRR29755808_2.fastq.gz	Fastqsanger.gz
	SRR29755809_1.fastq.gz	Fastqsanger.gz
	SRR29755809_2.fastq.gz	Fastqsanger.gz
	SRR29755810.fastq.gz	Fastqsanger.gz

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The role of KLF4 in the development of human primordial germ cell

Project description:Primordial germ cells (PGCs) are the founder cells that develop into mature gametes. PGCs emerge during weeks 2-3 of human embryo development. Pluripotency genes are reactivated during PGCs specification, including Kruppel-like factor KLF4, but its precise role in PGC development is unknown. Here, we investigated the role of KLF4 in PGC development using our in vitro model for human PGC-like cells (hPGCLCs). We show that depletion of KLF4 using acute protein degradation strategies reduced the efficiency of hPGCLC specification. We conducted transcriptome analysis of KLF4-depleted hPGCLCs, and observed downregulation of genes involved in pattern specification, stem cell differentiation, and epigenetic modifications. Notably, KLF4 depletion led to the derepression of some somatic genes related to endodermal and neuronal differentiation. In PGCLCs, the KLF4 Cut&Run results showed significant co-enrichment with SP and STAT motifs, prominently localized to promoter regions. This represents a distinct binding pattern compared to that observed in naive embryonic stem cells. Our study reveals that KLF4 contributes to the differentiation and maintenance of human PGCLCs by comprehensively regulating both gene activation and repression.

2025-01-29 | GSE271818 | GEO

Role of Dppa3 during primordial germ cell development I

Project description:Role of Dppa3 during primordial germ cell development I

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Role of Dppa3 during primordial germ cell development II

Project description:Role of Dppa3 during primordial germ cell development II

| PRJNA839021 | ENA

Divergent roles of KLF4 and TFCP2L1 in ground-state naive pluripotency and human primordial germ cell development

Project description:During development, human primordial germ cells (hPGCs) transition through a transcriptional and epigenetic state similar to pre-implantation epiblast cells called naive ground-state pluripotency. Diagnostic transcription factors that define this state include TFAP2C, KLF4, and TFCP2L1, with TFAP2C necessary for both establishment of the naive-like state in hPGC-like cells (hPGCLCs) as well as establishment and self-renewal of naive human embryonic stem cells (hESCs). Here, we show that KLF4 and TFCP2L1 are not required for hPGC specification or establishment of the naive-like state in hPGCLCs. Instead, KLF4 and TFCP2L1 are each required for reversion of primed hESCs to the self-renewing naive ground state. Additionally, TFCP2L1 but not KLF4 function after hPGC specification in the proliferation of the hPGCLC population.

2022-04-30 | GSE148717 | GEO

NRF1 promotes primordial germ cell development, proliferation, and survival [RNA-seq]

Project description:To investigate the role of NRF1 in regulating primordial germ cell development, We established conditional knockout mice of Nrf1 in primordial germ cell to observe the effect of Nrf1 knockout on the development of primordial germ cell. At the same time, we utilized a pluripotent stem cell differentiation system in vitro to obtain PGCL cells for chip_ Seq, analyze which genes Nrf1 directly binds to. Meanwhile, we established a pluripotent stem cell line induced by Nrf1 overexpression and performed RNA_seq analysis on PGCL cells overexpressing Nrf1 obtained in vitro

2023-08-05 | GSE231662 | GEO

NRF1 promotes primordial germ cell development, proliferation and survival [ChIP-seq]

2023-08-05 | GSE231661 | GEO

Role of Dppa3 during primordial germ cell development I

Project description:We performed whole-genome bisulfite sequencing and RNA-seq analysis of Stella knockout primordial germ cells (PGCs).

2023-04-27 | GSE196620 | GEO

Divergent roles of KLF4 and TFCP2L1 in ground-state naive pluripotency and human primordial germ cell development

Project description:Divergent roles of KLF4 and TFCP2L1 in ground-state naive pluripotency and human primordial germ cell development

| PRJNA625486 | ENA

NRF1 promotes primordial germ cell development, proliferation, and survival

Project description:NRF1 promotes primordial germ cell development, proliferation, and survival

| PRJNA967266 | ENA

In vitro modeling of primordial germ cell development

Project description:Transgenic StellaGFP ESCs were used to derive primordial germ cells during embryoid body (EB) differentiation, and microarry analysis used to compared FACS sorted Stella-positive cells of day 7 Ebs with the parental ESCs and Stella-negative cells of day 7 Ebs. Keywords: in vitro, primordial germ cells, embryonic stem cells, stella

2009-06-22 | GSE7948 | GEO

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