Project description:The study performed whole genome microarray gene expression profiling on primary human keratinoctyes cultured on collagen I, adult dermal fibroblast-derived ECM or foetal dermal fibroblast-derived ECM. Our gene array data showed that primary human keratinocytes grown on foetal dermal ECM upregulated genes involved in cell cycle, whereas on adult dermal ECM, a mix of differentiation and cell cycle genes were upregulated.
Project description:Transcript profiles of H. annosum from mycelium grown on different substrates and under different stresses were analyzed. The array probes were designed from gene models taken from the Joint Genome Institute (JGI, department of energy) H. irregulare genome sequence version 1. One aim of this study was to compare gene expression profiles of H. annosum pre-grown on secondary metabolites from the biocontrol agent Phlebiopsis gigantea.
Project description:Transcript profiles of H. annosum from different tissues and mycelium grown on different substrates and under different stresses were analyzed. The array probes were designed from gene models taken from the Joint Genome Institute (JGI, Department of Energy) H. irregulare genome sequence version 1. One aim of this study was to compare gene expression profiles of H. annosum grown on different substrates and under different biotic and abiotic stresses.
Project description:Transcript profiles of Heterobasion irregulare from different tissues and mycelium grown on different substrates were analyzed. The array probes were designed from gene models taken from the Joint Genome Institute (JGI, department of energy) Heterobasidion annosum genome sequence version 1. One aim of this study was to verify the expression of the automatically annotated gene models under various conditions. Another goal was to compare gene expression profiles from different tissues of Heterobasidion irregulare and from mycelium grown on liquid MMN medium, liquid medium amended with lignin or cellulose and on wood.
Project description:Transcript profiles of Serpula lacrymans grown on different substrates were analyzed. The array probes were designed from gene models taken from the Joint Genome Institute (JGI, department of energy) Serpula lacrymans genome sequence version 1. One aim of this study was to verify the expression of the automatically annotated gene models under various conditions. Another goal was to compare gene expression profiles from Serpula lacrymans grown on liquid MMN medium and on wood.
Project description:Transcript profiles of H. annosum from mycelium grown on different substrates and under different stresses were analyzed. The array probes were designed from gene models taken from the Joint Genome Institute (JGI, department of energy) H. irregulare genome sequence version 1. One aim of this study was to compare gene expression profiles of H. annosum pre-grown on secondary metabolites from the biocontrol agent Phlebiopsis gigantea. We performed six hybridizations with samples derived from H. annosum grown in either liquid Malt Extract Medium (three biological replicates) or on secondary metabolite from Phlebiopsis gigantea (three biological replicates). The Heterobasidion irregulare custom-exon expression array (4 x 72K) manufactured by Roche NimbleGen Systems Limited (Madison, WI) (http://www.nimblegen.com/products/exp/index.html) contained five independent, non-identical, 60-mer probes per gene model coding sequence. For 12,199 of the 12,299 annotated protein-coding gene models, probes could be designed. For 19 gene models, no probes could be generated, and 81 gene models shared all five probes with other gene models. Included in the array were 916 random 60-mer control probes and labelling controls. For 2032 randomly chosen gene models, technical duplicates were included on the array.