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ABSTRACT:
PROVIDER: PRJNA1136654 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Characterization of microbial communities at the genomic, transcriptomic, proteomic and metabolomic levels, with a special interest on lipid accumulating bacterial populations, which are naturally enriched in biological wastewater treatment systems and may be harnessed for the conversion of mixed lipid substrates (wastewater) into biodiesel. The project aims to elucidate the genetic blueprints and the functional relevance of specific populations within the community. It focuses on within-population genetic and functional heterogeneity, trying to understand how fine-scale variations contribute to differing lipid accumulating phenotypes. Insights from this project will contribute to the understanding the functioning of microbial ecosystems, and improve optimization and modeling strategies for current and future biological wastewater treatment processes. This project contains datasets derived from the same biological wastewater treatment plant. The data includes metagenomes, metatranscriptomes, metaproteomes and organisms isolated in pure cultures. Characterization of microbial communities at the genomic, transcriptomic, proteomic and metabolomic levels, with a special interest on lipid accumulating bacterial populations, which are naturally enriched in biological wastewater treatment systems and may be harnessed for the conversion of mixed lipid substrates (wastewater) into biodiesel. The project aims to elucidate the genetic blueprints and the functional relevance of specific populations within the community. It focuses on within-population genetic and functional heterogeneity, trying to understand how fine-scale variations contribute to differing lipid accumulating phenotypes. Insights from this project will contribute to the understanding the functioning of microbial ecosystems, and improve optimization and modeling strategies for current and future biological wastewater treatment processes. This project contains datasets derived from the same biological wastewater treatment plant. The data includes metagenomes, metatranscriptomes, metaproteomes and organisms isolated in pure cultures.
2020-08-19 | PXD013655 | Pride
Project description:In this study we linked the biological end point of genomic DNA damage from our quantitative, comparative disinfection by-product (DBP) database, with toxicogenomic analysis using a Super Array RT2 Profiler™ PCR Array containing primers for 84 genes related to human DNA damage and repair and 84 genes whose expression level is indicative of stress and toxicity.
2009-04-08 | GSE15488 | GEO
Project description:Effect of chlorination on the toxicity of wastewater effluents treated by activated sludge (AS) and submerged membrane bioreactor (S-MBRB) systems to HepG2 human hepatoblastoma cells was investigated. In addition to cytotoxicity assay, the DNA microarray-based transcriptome analysis was performed to evaluate the change in modes of toxic actions (MOAs) of effluents by chlorination. Effluent organic matters (EfOM) and disinfection by-products (DBPs) were characterized by using Fourier transform mass spectrometry (FT-MS). The cytotoxicity of AS effluent was elevated by chlorination, while the toxicity of S-MBRB effluent was reduced. The averaged O/C ratio of EfOM in S-MBRB effluent was lower than that in AS effluent. The results of the transcriptome and FT-MS analyses suggested that lower O/C molecules influenced on M-bM-^@M-^\response to hormone stimulusM-bM-^@M-^] and M-bM-^@M-^\acute inflammatory responseM-bM-^@M-^] but those were decreased by chlorination, which consequently reduced cytotoxicity. On the other hand, larger number of DBPs and other molecules were increased in AS effluents by chlorination. Those molecules might influence on M-bM-^@M-^\cellular metabolic processM-bM-^@M-^], which consequently elevated cytotoxicity. Therefore, the combination of the toxicity assays and chemical analysis demonstrated the changes in severity of cytotoxicity and MOAs by chlorination, and the difference of chemical characteristics which relate to those toxicity changes. We examined the gene expression alteration in human hepatoma cell line, HepG2 exposed to the chlorinated wastewater effluents from membrane bioreactor and the activated sludge process. Human Genome Focus Array, which represents 8,795 verified human sequences, was used. All effluent samples were concentrated by using solid phase extraction (SPE). SPE fraction from MQ water was used as controll. For duplicate, two dishes were prepared for each sample and individually treated in parallel.
2013-06-21 | E-GEOD-48157 | biostudies-arrayexpress
Project description:We report the transcriptomic changes in Salmonella Typhimurium exposed to sub-lethal sonophotocatalytic disinfection. The current data suggests that more than 120 genes are significantly expressed during the process. The genes associated with the flagellar assembly were found to be significantly up-regulated during the disinfection, which may have impacts on the phenotypic attributes of the bacteria.
2024-07-29 | GSE181112 | GEO
Project description:Our study revealed a synergistic effect between biological nitrogen fixation and current generation by G. sulfurreducens, providing a green nitrogen fixation alternative through shifting the nitrogen fixation field from energy consumption to energy production and having implications for N-deficient wastewater treatment.
2021-10-09 | GSE185414 | GEO
Project description:One of the most widely used drugs in municipal wastewater treatment effluents and soil is carbamazepine, a commonly prescribed antidepressants and antiepileptic drug. Carbamazepine exerts an intrinsic biological activity on the nervous system, thus may induce ecotoxicological effects on non-target organisms. Earthworms, one of the essential indicator species of soil health, accumulate biosolid fertilisers and wastewater contaminants. In this project, earthworms (Dendrobaena veneta) were treated with carbamazepine to explore their uptake dynamics, molecular and life cycle endpoints. By conducting transcriptomic profiling of different tissues in an organism exposed to carbamazepine assists in defining detoxification and neural system responses in the terrestrial invertebrate.
2025-05-21 | GSE268697 | GEO
Project description:Manufactured nanomaterials (MNMs) are increasingly incorporated into consumer products that are disposed into sewage. In wastewater treatment, MNMs adsorb to activated sludge biomass where they may impact biological wastewater treatment performance, including nutrient removal. Here, we studied MNM effects on bacterial polyhydroxyalkanoate (PHA), specifically polyhydroxybutyrate (PHB), biosynthesis because of its importance to enhanced biological phosphorus (P) removal (EBPR). Activated sludge was sampled from an anoxic selector of a municipal wastewater treatment plant (WWTP), and PHB-containing bacteria were concentrated by density gradient centrifugation. After starvation to decrease intracellular PHB stores, bacteria were nutritionally augmented to promote PHB biosynthesis while being exposed to either MNMs (TiO2 or Ag) or to Ag salts (each at a concentration of 5 mg L-1). Cellular PHB concentration and PhyloChip community composition were analyzed. The final bacterial community composition differed from activated sludge, demonstrating that laboratory enrichment was selective. Still, PHB was synthesized to near-activated sludge levels. Ag salts altered final bacterial communities, although MNMs did not. PHB biosynthesis was diminished with Ag (salt or MNMs), indicating the potential for Ag-MNMs to physiologically impact EBPR through the effects of dissolved Ag ions on PHB producers.
2014-12-03 | GSE63781 | GEO
Project description:Effect of soil disinfection with chemical and biological methods on bacterial communities
| PRJDB4211 | ENA