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ABSTRACT:
PROVIDER: PRJNA1140525 | ENA |
REPOSITORIES: ENA
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Project description:This study investigates the role of carbon-to-phosphorus (C/P) ratios in shaping microbial community dynamics and polyhydroxyalkanoates (PHA) production in sequencing batch reactors (SBR) fed with volatile fatty acids. Three conditions, characterized by fixed organic loading rates but varying C/P ratios (Run 1 = 170 Cmol Pmol-1; Run 2 = 235 Cmol Pmol-1; Run 3 = 400 Cmol Pmol-1), were tested to explore their impact on PHA accumulation, biomass growth, and reactor stability. Results indicate that the moderate phosphorus limitation of Run 2 achieves the best overall performance, with a PHA volumetric productivity of 2.02 g PHA L-1 d-1 and process stability. Conversely, higher C/P ratio increased the storage yield but lowered the productivity (1.55 gPHA L-1 d-1), compromising sludge settleability and reactor stability, indicating impaired microbial functionality. Full-length 16S/18S rRNA gene sequencing using PacBio technology enabled high-resolution profiling of microbial communities, revealing ecological shifts across conditions. Run 2 exhibited the highest bacterial and eukaryotic diversity, featuring multiple PHA-accumulating bacteria (mainly Sphaerotilus, Leadbetterella, and uncultured Rhodobacteraceae) and a well-structured eukaryotic community dominated by K-strategist bacterivorous protists, e.g. Rhogostoma (Rhizaria) and Vorticella (Ciliophora, Oligohymenophorea) and predatory protozoa, e.g. Tokophrya (Ciliophora, Suctoria). In contrast, Sphaerotilus dominated under suboptimal conditions and may be linked to bulking. These results suggest that eukaryotic diversity may support system stability through selective predation. Transmission electron microscopy (TEM) further confirmed the presence of intracellular PHA granules and polyphosphate reserves, reinforcing the connection between nutrient limitation and adaptive microbial strategies. Overall, these findings highlight the critical role of the C/P ratio in shaping the performance of mixed microbial cultures, demonstrating that a well-balanced nutrient supply can enhance PHA production while maintaining microbial community stability. The results contribute to optimizing the selection process for mixed microbial cultures, offering valuable insights into the impact of carbon-to-nutrient ratios in the feeding strategy.
2025-07-14 | GSE302521 | GEO
Project description:This study investigates the role of carbon-to-phosphorus (C/P) ratios in shaping microbial community dynamics and polyhydroxyalkanoates (PHA) production in sequencing batch reactors (SBR) fed with volatile fatty acids. Three conditions, characterized by fixed organic loading rates but varying C/P ratios (Run 1 = 170 Cmol Pmol-1; Run 2 = 235 Cmol Pmol-1; Run 3 = 400 Cmol Pmol-1), were tested to explore their impact on PHA accumulation, biomass growth, and reactor stability. Results indicate that the moderate phosphorus limitation of Run 2 achieves the best overall performance, with a PHA volumetric productivity of 2.02 g PHA L-1 d-1 and process stability. Conversely, higher C/P ratio increased the storage yield but lowered the productivity (1.55 gPHA L-1 d-1), compromising sludge settleability and reactor stability, indicating impaired microbial functionality. Full-length 16S/18S rRNA gene sequencing using PacBio technology enabled high-resolution profiling of microbial communities, revealing ecological shifts across conditions. Run 2 exhibited the highest bacterial and eukaryotic diversity, featuring multiple PHA-accumulating bacteria (mainly Sphaerotilus, Leadbetterella, and uncultured Rhodobacteraceae) and a well-structured eukaryotic community dominated by K-strategist bacterivorous protists, e.g. Rhogostoma (Rhizaria) and Vorticella (Ciliophora, Oligohymenophorea) and predatory protozoa, e.g. Tokophrya (Ciliophora, Suctoria). In contrast, Sphaerotilus dominated under suboptimal conditions and may be linked to bulking. These results suggest that eukaryotic diversity may support system stability through selective predation. Transmission electron microscopy (TEM) further confirmed the presence of intracellular PHA granules and polyphosphate reserves, reinforcing the connection between nutrient limitation and adaptive microbial strategies. Overall, these findings highlight the critical role of the C/P ratio in shaping the performance of mixed microbial cultures, demonstrating that a well-balanced nutrient supply can enhance PHA production while maintaining microbial community stability. The results contribute to optimizing the selection process for mixed microbial cultures, offering valuable insights into the impact of carbon-to-nutrient ratios in the feeding strategy.
2025-07-14 | GSE302519 | GEO
Project description:This study investigates the role of carbon-to-phosphorus (C/P) ratios in shaping microbial community dynamics and polyhydroxyalkanoates (PHA) production in sequencing batch reactors (SBR) fed with volatile fatty acids. Three conditions, characterized by fixed organic loading rates but varying C/P ratios (Run 1 = 170 Cmol Pmol-1; Run 2 = 235 Cmol Pmol-1; Run 3 = 400 Cmol Pmol-1), were tested to explore their impact on PHA accumulation, biomass growth, and reactor stability. Results indicate that the moderate phosphorus limitation of Run 2 achieves the best overall performance, with a PHA volumetric productivity of 2.02 g PHA L-1 d-1 and process stability. Conversely, higher C/P ratio increased the storage yield but lowered the productivity (1.55 gPHA L-1 d-1), compromising sludge settleability and reactor stability, indicating impaired microbial functionality. Full-length 16S/18S rRNA gene sequencing using PacBio technology enabled high-resolution profiling of microbial communities, revealing ecological shifts across conditions. Run 2 exhibited the highest bacterial and eukaryotic diversity, featuring multiple PHA-accumulating bacteria (mainly Sphaerotilus, Leadbetterella, and uncultured Rhodobacteraceae) and a well-structured eukaryotic community dominated by K-strategist bacterivorous protists, e.g. Rhogostoma (Rhizaria) and Vorticella (Ciliophora, Oligohymenophorea) and predatory protozoa, e.g. Tokophrya (Ciliophora, Suctoria). In contrast, Sphaerotilus dominated under suboptimal conditions and may be linked to bulking. These results suggest that eukaryotic diversity may support system stability through selective predation. Transmission electron microscopy (TEM) further confirmed the presence of intracellular PHA granules and polyphosphate reserves, reinforcing the connection between nutrient limitation and adaptive microbial strategies. Overall, these findings highlight the critical role of the C/P ratio in shaping the performance of mixed microbial cultures, demonstrating that a well-balanced nutrient supply can enhance PHA production while maintaining microbial community stability. The results contribute to optimizing the selection process for mixed microbial cultures, offering valuable insights into the impact of carbon-to-nutrient ratios in the feeding strategy.
2025-07-14 | GSE302520 | GEO
Project description:A three-stage continuous fermentative system was developed to simulate and control physicochemical factors of the gut biology. Inoculation was of each reactor was performed from a human fecal sample which was initially amplified with a batch procedure. Samples from the initial feces, the batch and from the bioreactors media were collected to extract bacterial DNA. 16S PCR amplification was performed to assess the microbial diversity at the family level using the HuGChip. Amplified DNA was purified and labelled with either Cy3 or Cy5 dye and hybridized on the microarray.
2013-05-07 | GSE46677 | GEO
Project description:A three-stage continuous fermentative system was developed to simulate and control physicochemical factors of the gut biology. Inoculation was of each reactor was performed from a human fecal sample which was initially amplified with a batch procedure. Samples from the initial feces, the batch and from the bioreactors media were collected to extract bacterial DNA. 16S PCR amplification was performed to assess the microbial diversity at the family level using the HuGChip. Amplified DNA was purified and labelled with either Cy3 or Cy5 dye and hybridized on the microarray. A 5 chip study was realized, each corresponding to hybridization with 250ng of labelled 16S rRNA gene amplicons from either the initial stool, the batch inoculum or fermentative medium different compartments of the simulated colon (Proximal, Transversal and Distal). Each probe (4441) was synthetized in three replicates.
2013-05-07 | E-GEOD-46677 | biostudies-arrayexpress