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Effect of Overexpression of DPP4 on Gene Expression in C28/I2 Chondrocyte Cells

ABSTRACT: Effect of Overexpression of DPP4 on Gene Expression in C28/I2 Chondrocyte Cells

PROVIDER: PRJNA1141116 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30013912_1.fastq.gz	Fastqsanger.gz
	SRR30013912_2.fastq.gz	Fastqsanger.gz
	SRR30013913_1.fastq.gz	Fastqsanger.gz
	SRR30013913_2.fastq.gz	Fastqsanger.gz
	SRR30013914_1.fastq.gz	Fastqsanger.gz

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Effect of Overexpression of DPP4 on Gene Expression in C28/I2 Chondrocyte Cells

Project description:We report here the overexpression of DPP4 in C28/I2 chondrocyte cells to investigate the changes induced by elevated levels of this gene. DPP4 is known as a marker of cellular senescence, yet its regulatory mechanisms remain unclear. In this study, we utilized the C28/I2 cell line to overexpress DPP4 and analyzed the resultant effects on gene expression and cellular behavior. Our findings reveal that DPP4 overexpression influences several pathways associated with chondrocyte function and senescence. Specifically, we observed alterations in pathways related to extracellular matrix organization, inflammatory response, and cellular aging. These results suggest that DPP4 plays a significant role in the modulation of chondrocyte senescence and may provide new insights into the mechanisms of cellular aging. Taken together, our data provide a molecular framework for understanding how DPP4 overexpression impacts chondrocyte physiology and offers potential targets for therapeutic intervention in age-related cartilage diseases.

2025-02-19 | GSE273261 | GEO

Gene expression analysis in C28/I2 cells: Comparison of OENC, DPP4 Overexpression, and DPP4 Overexpression with 4,5-Dicaffeoylquinic Acid Treatment Using RNA-Seq

Project description:To examine the effects of DPP4 overexpression and its modulation by 4,5-Dicaffeoylquinic acid on chondrocytes, we established C28/I2 cell lines with DPP4 overexpression (DPP4OE), control empty vector (OENC), and DPP4OE treated with 4,5-Dicaffeoylquinic acid. Gene expression profiling analysis was performed using RNA sequencing (RNA-seq) with biological replicates to investigate the global transcriptomic changes associated with these conditions.

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Effects of piR_019914 on gene expression in C28/I2 cells

Project description:In order to study the role of piR_019914 in C28/I2 cells, we established piR_019914_mimics to overexpress piR_019914 in C28/I2 cells We then performed gene expression profiling analysis using data obtained from RNA-seq of piR_019914 overexpression C28/I2 cells and mimics_NC C28/I2 cells.

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Gene expression analysis in C28/I2 cells: Comparison of OENC, DPP4 Overexpression, and DPP4 Overexpression with 4,5-Dicaffeoylquinic Acid Treatment Using RNA-Seq

Project description:Gene expression analysis in C28/I2 cells: Comparison of OENC, DPP4 Overexpression, and DPP4 Overexpression with 4,5-Dicaffeoylquinic Acid Treatment Using RNA-Seq

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Gene expression of C28/I2 cells with FABP4

Project description:Gene expression of C28/I2 cells with FABP4

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Effects of piR_019914 on gene expression in C28/I2 cells

Project description:Effects of piR_019914 on gene expression in C28/I2 cells

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Gene expression of C28/I2 cells in monolayer and spheroid culture

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CircRNA-MSR Regulates LPS-Induced C28/I2 Chondrocyte Injury through miR-643/MAP2K6 Signaling Pathway.

Project description:ObjectiveOsteoarthritis (OA) is a degenerative joint disease characterized by deterioration of articular cartilage functions. Previous studies have confirmed the role of circular RNAs (circRNAs) in OA, but the role of mechanical stress-related circRNA (circRNA-MSR) in OA is unknown.DesignThe human chondrocytes C28/I2 were cultured and treated with lipopolysaccharide (LPS) to establish the OA model. The mRNA and protein levels were measured by qRT-PCR or Western blot. Cell viability was analyzed by MTT assay. Flow cytometry was carried out to detect cell apoptosis. The levels of TNF-α, IL-1β, and IL-6 were determined by enzyme-linked immunosorbent assay (ELISA). Pull-down assay was conducted to measure circRNA-MSR-related miRNA. Dual-luciferase reporter gene detection was performed to detect the target relationships between miR-643 and circRNA-MSR or Mitogen-activated protein kinase kinase 6 (MAP2K6). The RNA-fluorescence in situ hybridization (RNA-FISH) assay was conducted to verify the localization of circRNA-MSR and miR-643.ResultsThe expressions of circRNA-MSR were upregulated in LPS stimulated C28/I2 cells. Knockdown of circRNA-MSR can inhibit LPS-induced apoptosis, inflammatory response, and extracellular matrix (ECM) degradation, and promote cell C28/I2 cells proliferation. Moreover, circRNA-MSR directly targeted miR-643. RNA-FISH exhibited that circRNA-MSR may act as a competing endogenous RNA (ceRNA) of miR-643. Over-expression of miR-643 could alleviate LPS-induced C28/I2 chondrocyte injury and promote cell proliferation. Besides, miR-643 directly bound to MAP2K6 mRNA. MiR-643 inhibition or MAP2K6 overexpression can reverse the role of circRNA-MSR knockdown on LPS-treated chondrocytes.ConclusioncircRNA-MSR can upregulate MAP2K6 by targeting miR-643, thereby inhibiting cell proliferation and promoting apoptosis of C28/I2 cells.

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Identification of C9orf72 as a potential target of osteoarthritis via STING/NF-κB axis by integrating bioinformatics and experiments

Project description:RNA-sequence analysis demonstrated ECM degradation and inflammation activation in C28/I2 cells with C9orf72 knockdown

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