Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Multiway chromatin conformation analyses at the human immunoglobulin heavy chain locus reveal regulatory principles underlying somatic hypermutation [Tri-C]

ABSTRACT: Multiway chromatin conformation analyses at the human immunoglobulin heavy chain locus reveal regulatory principles underlying somatic hypermutation [Tri-C]

PROVIDER: PRJNA1141913 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30035800_1.fastq.gz	Fastqsanger.gz
	SRR30035800_2.fastq.gz	Fastqsanger.gz
	SRR30035801_1.fastq.gz	Fastqsanger.gz
	SRR30035801_2.fastq.gz	Fastqsanger.gz
	SRR30035802_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 106

Similar Datasets

Regulation of somatic hypermutation by higher-order chromatin structure [Tri-C]

Project description:The generation of protective antibodies by somatic hypermutation (SHM) is essential for antibody maturation and adaptive immunity. SHM involves co-transcriptional mutagenesis of immunoglobulin variable (V) regions regulated by enhancers located hundreds of kilobases away. How 3D chromatin structure affects SHM is poorly understood. Here, we measure higher-order interactions on single alleles of the human immunoglobulin heavy chain locus (IGH) using Tri-C. We find that SHM is underpinned by a multiway hub wherein the V region is proximal to all enhancers. Cohesin-mediated loop extrusion is dispensable for IGH transcription and hub architecture. Transcription and mutagenesis of IGH switch regions, which are necessary for antibody class-switch recombination, creates new chromatin loops that can form without cohesin. However, these additional loops do not compromise hub integrity, V region transcription or SHM. Thus, antibody maturation occurs within a multiway hub accommodating several gene-enhancer loops wherein transcription and mutagenesis of different segments can occur non-competitively.

2025-06-20 | GSE273433 | GEO

Regulation of somatic hypermutation by higher-order chromatin structure

Project description:Regulation of somatic hypermutation by higher-order chromatin structure

| PRJNA1141910 | ENA

Regulation of somatic hypermutation by higher-order chromatin structure

Project description:Regulation of somatic hypermutation by higher-order chromatin structure

| PRJNA1132192 | ENA

Regulation of somatic hypermutation by higher-order chromatin structure

2025-06-16 | GSE271541 | GEO

Regulation of somatic hypermutation by higher-order chromatin structure [PRO-Seq]

2025-06-16 | GSE273431 | GEO

Regulation of somatic hypermutation by higher-order chromatin structure [ChIP-Seq]