Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1142934 | ENA |
REPOSITORIES: ENA
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Project description:Monoclonal antibodies are widely used to treat follicular lymphoma (FL) and multiple myeloma (MM), but prolonged exposure may impair immune function and increase infection risk. Thus, probiotics are emerging as a strategy to counteract pathogenic colonization. Among them, Streptococcus salivarius 24SMB emerges as a promising oral probiotic. In this project, its beneficial activity was investigated using the amoeba model system Dictyostelium discoideum, widely used for studying host–bacteria interactions. Dictyostelium can be considered as a primitive macrophage, able to feed on bacteria. It was used to evaluate the effects of S. salivarius and the oral pathogen Streptococcus mutans through growth, viability, chemotaxis and phagocytosis assays, combined with transcriptomic and metabolomic analyses. Both S. salivarius and S. mutans reduced Dictyostelium growth compared to E. coli (control). However, S. salivarius significantly improved viability, particularly in sequential exposure experiments, where pre-culture with S. salivarius followed by S. mutans increased vitality by 30%. Moreover, while S. mutans reduced Dictyostelium chemotactic competence, S. salivarius enhanced it. Pre-culture with S. salivarius also increased phagocytosis of oral pathogens (S. mutans and E. corrodens), confirmed by higher intracellular ROS. Transcriptomic and metabolomic analyses further support obtained results, indicating a more active metabolic and phagocytic-primed state after S. salivarius-exposure. In contrast, S. mutans induced a stressed, energy-depleted profile. In conclusion, S. salivarius primed innate immune functions, enhancing Dictyostelium viability, chemotaxis and phagocytic capacity. These findings support Dictyostelium as a simple, cost-effective model for probiotic screening and pathogen-induced stress response studies.
2026-07-06 | GSE337573 | GEO
Project description:To investigate the subspecies level differences in the expression profiles of Streptococcus salivarius strains, we conducted the RNA sequencing analysis of Streptococcus salivarius strains.
2024-11-10 | GSE278791 | GEO
Project description:The Type VI Secretion System (T6SS) in bacteria is a versatile mechanism that facilitates protein transport into neighboring cells and can act as an antibacterial weapon by eliminating competing organisms in the vicinity. The objective of this study was to characterize the T6SS in Aggregatibacter aphrophilus and assess its antimicrobial capabilities through competition with Aggregatibacter actinomycetemcomitans in a multispecies biofilm. The proteomic analysis consisted of two parts, referred to as monospecies biofilm and multispecies biofilms, respectively. Initially, we examined the protein profiles of monospecies biofilms formed by two strains of Aggregatibacter aphrophilus, namely HK83 and CCUG 11575, along with their Hcp mutant derivatives (Hcp being a core protein for T6SS). Each strain was analyzed with six replicates (n=4 for HK83, HK83 hcp, CCUG 11575, and CCUG 11575 hcp). Subsequently, the HK83 and CCUG 11575 strains, as well as their Hcp mutant derivatives, were individually introduced into a multispecies biofilm. This multispecies biofilm consisted of seven species, namely A. actinomycetemcomitans JP2 strain (OMZ 295), Actinomyces oris (OMZ 745), Candida albicans (OMZ 110), Fusobacterium nucleatum subsp. nucleatum KP-F2 (OMZ 598), Streptococcus oralis SK248 (OMZ 607), Streptococcus mutans UA159 (OMZ 918), and Veillonella dispar ATCC 17748T (OMZ 493). These species were selected to mimic the natural co-habitat of A. aphrophilus and A. actinomycetemcomitans. Furthermore, control 7-species biofilms with A. aphrophilus strains HK83, HK83 hcp, CCUG 11575, and CCUG 11575 hcp (n=4 each) underwent proteomic analysis to gain insights into the protein expression and potential interactions within the biofilm community.
2024-05-24 | PXD042723 | Pride
Project description:The influence of cranberry proanthocyanidins on the transcriptomic responses of Streptococcus mutans during biofilm formation was investigated. Treatment regimens simulating topical exposures experienced clinically (twice-daily, 60 s each) were used over saliva-coated hydroxyapatite biofilm model. Cranberry proanthocyanidins (1.5 mg/ml) in 15% ethanol was used to treat the biofilms. Four biological replicates each for the treatment and vehicle control were used for RNA extraction and microarray.
2012-08-22 | E-GEOD-40172 | biostudies-arrayexpress
Project description:NIP and NrpR forms a pair for the transcriptional regulator in Streptococcus salivarius K12 (SAL). To explore the target genes regulated by the NIP-NrpR pair in Streptococcus salivarius K12 (SAL), we generated mutants in which the NIP or NrpR gene has been knocked down through complement recombination. Comparative transcriptome profiling of WT, ▲nrpR, and nip* mutant strains revealed that the sar BGC is the major regulatory target controlled by the NIP signaling pathway in SAL.
2023-11-18 | GSE247581 | GEO
Project description:genome of oral probiotic Streptococcus salivarius 24SMBc
| PRJNA865118 | ENA
Project description:The manufacturing processes of commercial probiotic strains may be affected in different ways in the attempt to optimize yield, costs, functionality, or stability, influencing gene expression, protein patterns, or metabolic output. Aim of this work was to compare different samples of a high concentration (450 billion bacteria) multispecies (8 strains) formulation produced at two different manufacturing sites, USA (US) and Italy (IT), by applying functional proteomics analyses. Several protein-profile differences were detected between IT- and US-made products, with Lactobacillus paracasei, Streptococcus thermophilus, and Bifidobacteria being the main affected probiotics/microorganisms. Integration of omics platforms with in vivo analysis emerged as a powerful tool to assess manufacturing procedures.
2020-01-22 | PXD017086 | Pride
Project description:Chlorhexidine (CHX) is a widely used antiseptic agent in dental care due to its broad-spectrum antimicrobial properties. This study focuses on the transcriptomic changes associated with chlorhexidine adaptation in oral Streptococcus salivarius (73_wt, 73_a), Streptococcus vestibularis (78_wt, 78_e), and Streptococcus mitis (93_wt, 93_d) using RNA sequencing.
2024-12-31 | GSE277379 | GEO