Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Enhanced CLIP-Seq (eCLIP-Seq) of FLAG-tagged wild-type and mutant MYC in U2OS-MYC-KO-MYC-Tet-On cells

ABSTRACT: Enhanced CLIP-Seq (eCLIP-Seq) of FLAG-tagged wild-type and mutant MYC in U2OS-MYC-KO-MYC-Tet-On cells

PROVIDER: PRJNA1143038 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

FLAG-tagged FOXM1A Plasmid

Project description:FLAG-tagged FOXM1A Plasmid

| PRJEB24606 | ENA

Enhanced CLIP-Seq (eCLIP-Seq) of FLAG-tagged wild-type and mutant MYC in U2OS-MYC-KO-MYC-Tet-On cells

Project description:Transcription factor (TF) MYC binds to thousands of gene regulatory elements in the genome such as promoters and enhancers and exerts an amplification effect on the expression of most genes, regardless of the E-box motif existence. In this study, we discovered that MYC is an RNA-binding protein with a high affinity to guanosine-rich RNAs. RNAs that bind to MYC increase MYC’s chromatin occupancy, which helps with MYC-mediated regulation of gene expression. Mechanistically, two highly conserved sequences, amino acids 355-357 and 364-367, in the basic region of MYC are essential for RNA binding. Notably, alanine substitution of Lys355, Arg356, and Arg357 of MYC abolishes its RNA binding function but does not influence its complex formation ability, and E-box binding capacity of MYC:MAX dimer in vitro. However, loss of RNA binding function alone decreases MYC recruitment at gene regulatory elements in vivo, followed by a decreased MYC:MAX chromatin binding at these regions. This process further attenuates MYC-mediated gene expression. Therefore, RNA-binding-deficient MYC is compromised in promoting cancer cell proliferation. By demonstrating the mechanism of MYC-RNA interaction and the fundamental role of RNA binding activity in MYC-centric functions, our study reveals a new paradigm to MYC function and highlights an essential and probably general role of RNA binding capacity of TFs for their gene regulatory function.

2025-05-08 | GSE273775 | GEO

FLAG-tagged hnRNPM iCLIP in AGS cells

Project description:FLAG-tagged hnRNPM iCLIP in AGS cells

| PRJNA737793 | ENA

Genome-wide maps of chromatin state in HCT116 cells expressing Flag-tagged VCP

Project description:Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for VCP and Flag in HCT116 cells expressing Flag-tagged VCP.

2023-07-18 | GSE201200 | GEO

ChIP-seq of MOZ (Flag-V5-BIO tagged) in mouse mouse craniofacial development

Project description:Carboxy-terminally tagged MOZ (Flag-V5-BIO tagged) was detected by ChIP-seq using anti-V5 antibody (Sigma, A7345) to precipitate chromatin associated with MOZ

2019-07-23 | GSE134607 | GEO

Chromatin immunoprecipitation of human HEK293 cells with flag-tagged IMP1 antibody

Project description:Isolation of IMP1 bound mRNAs. Flag-tagged IMP1 was expressed in HEK293 cells. Flag tagged IMP1 was immunoprecipitated and mRNAs isolated. As controls HEK293 cells that do not express Flag-tagged IMP1 was included.

2006-12-31 | E-MEXP-841 | biostudies-arrayexpress

Mapping the genomic occupancy by flag-tagged APOBEC3B protein

Project description:Mapping the genomic occupancy by flag-tagged APOBEC3B protein

| PRJNA795961 | ENA

RNA-sequencing of high grade glioma (HGG) human cells encoding for H3.3-G34R vs H3.3-WT histones, derived from a patient harboring pHGG and stably tranfected with 3X-FLAG-tagged wild type H3.3 or a 3X-FLAG-tagged H3.3 harboring the G34R mutation

Project description:RNA-sequencing of H3.3-G34R and H3.3-WT HGG cells was performed to uncover transcriptomic differences related to the presence of H3.3-G34R mutation, using human cells obtained from a patient harboring pHGG and stably tranfected with 3X-FLAG-tagged wild type H3.3 or a 3X-FLAG-tagged H3.3 harboring the G34R mutation.

2022-01-01 | GSE182068 | GEO

GRO-seq analysis of control and Myc-induced U2OS cells

Project description:We used GRO-seq to examine the effect of Myc activation on RNA transcription in U2OS cells. We measure in duplicates gene transcription rates in U2OS cells containing an inducible Myc expression vector that were induced or mock-treated in duplicates for 5 hours.

2015-11-04 | E-GEOD-66928 | biostudies-arrayexpress

Ribosome profiling and RNA sequencing of U2OS cells overexpressing myc

Project description:Ribosome profiling and RNA sequencing was performed on U2OS cells, either control or overexpressing myc.

2017-07-27 | GSE59819 | GEO