Project description:We performed bulk RNA-seq analysis of antennae from three notable pest ant species, Camponotus floridanus, Atta sexdens, and Atta cephalotes, in order to characterize caste-specific expression patterns of odorant receptor genes.
Project description:The database consists of 248 HPLC-QTOF-HRMS/MS analyses of microscopic fungi isolated from soil samples collected in 2021, in the cloud forest of central Veracruz, Mexico. Soil samples were collected from around of 17 ant nests, from inside of 15 anthills, and from two transit areas. Also entomogenic fungi of ants. The two best represented ants were Solenopsis geminata and Atta mexicana, the others were Dorymyrmex bicolor, Nomamyrmex esenbeckii, Cheliomyrmex morosus, and Camponotus sericeiventris. Growth media and solvents analyses are available.
Dataset license: CC BY-NC-ND 4.0
Project description:Long noncoding RNAs (lncRNAs), as a class of emerging regulators, play crucial role in regulating the strength and duration of innate immunity. However, little is known about how these Drosophila Imd immunity-related lncRNAs are regulated. Herein, we firstly revealed that overexpression of lncRNA-CR33942 could strengthen the expression of Imd pathway antimicrobial peptides Diptericin (Dpt) and Attacin-A (AttA) after infection, and vice versa. Secondly, RNA-seq analysis of post-infected lncRNA-CR33942-overexpressing flies further confirmed that lncRNA-CR33942 positively regulates the Drosophila Imd pathway. Mechanistically, we indicated that lncRNA-CR33942 interacts with NF-κB transcription factor Relish to promote its binding to Dpt and AttA promoters, thereby facilitating Dpt and AttA expression. Interestingly, we found that Relish can also directly promote lncRNA-CR33942 transcription by binding to its promoter. Finally, rescue experiments and dynamic expression profiling post-infection demonstrated the vital role of the Relish/lncRNA-CR33942/AMPs regulatory axis in enhancing inadequate Imd immune responses and maintaining immune homeostasis. Taken together, our study not only elucidates a novel mechanism about lncRNA in Drosophila Imd immune regulation, but also has important guiding significance for elucidating the complex regulatory mechanism of animal innate immune response.
Project description:To determine the modulation of gene expression of Leishmania mexicana(M379)-inoculated BALB/c ears in the presence of promastigote secretory gel (PSG) A genome-wide transcriptional analysis was performed by comparing the gene expression profiles of Leishmania mexicana- inoculated BALB/c ears and Leishmania mexicana plus PSG BALB/c ears. Leishmania mexicana amastigotes were purified from mouse cutaneous lesions and transformed in vitro in metacycic promastigotes (MT). After 6, 24 and 48 hours, ears were collected and processed for RNA extraction. Three Biological replicates per condition were run.