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Knockout CD45 in LatY136F mice affact T cell activation

ABSTRACT: Knockout CD45 in LatY136F mice affact T cell activation

PROVIDER: PRJNA1143418 | ENA |

REPOSITORIES: ENA

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Knockout CD45 in LatY136F mice affact T cell activation

Project description:Background: The adaptor protein Linker for activation of T cell (LAT) is a key signaling hub used by the T cell antigen receptor. Mice with a mutation at the LAT-PLCγ1 binding site (Y136) have a defect in thymocyte development due to dampened TCR signaling. CD4 + T cells that do reach the periphery are hyper-activated and skewed to Th2. Over time, these mice develop an autoimmune-like syndrome, characterize by overproduction of Th2 cytokines, T cell infiltration into various organs, and B cell activation, isotype switching, and autoantibody production. In this study, we established LatY136F and LatY136F-CD45 KO mice and using them to investigate to role of CD45 in TCR signalling via flow cytometry and scRNA sequencing. Conclusion: Knockout CD45 in LatY136F mice affact T cell activation

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