Project description:The study is done to compare the impact of high light on the photosynthetic bacterium Rhodobacter alkalitolerans strain JA916T when grown in high pH and neutral pH. After the analysis we found several photosynthetic and cell division transcripts to be differentially expressed. These transcripts were majorly from the bacteriochlorophyll and carotenoid biosynthesis pathway. Reaction center transcripts were also found to be highly upregulated in nhpH conditions. Similary cell division transcripts were also found to be highly upregulated in high light conditions. Further for detailed study the publication can be referred. This study is to be published in the article titled as " Integration of transcriptomics and metabolomics for understanding the global responses to neutral pH and high pH under high light in photosynthetic bacterium Rhodobacter alkalitolerans strain JA916T".
Project description:Many bacteria, often associated with eukaryotic hosts and of relevance for biotechnological applications, harbor a multipartite genome composed of more than one replicon. Biotechnologically relevant phenotypes are often encoded by genes residing on the secondary replicons. A synthetic biology approach to developing enhanced strains for biotechnological purposes could therefore involve merging pieces or entire replicons from multiple strains into a single genome. Here we report the creation of a genomic hybrid strain in a model multipartite genome species, the plant-symbiotic bacterium Sinorhizobium meliloti. We term this strain as cis-hybrid, since it is produced by genomic material coming from the same species' pangenome. In particular, we moved the secondary replicon pSymA (accounting for nearly 20% of total genome content) from a donor S. meliloti strain to an acceptor strain. The cis-hybrid strain was screened for a panel of complex phenotypes (carbon/nitrogen utilization phenotypes, intra- and extracellular metabolomes, symbiosis, and various microbiological tests). Additionally, metabolic network reconstruction and constraint-based modeling were employed for in silico prediction of metabolic flux reorganization. Phenotypes of the cis-hybrid strain were in good agreement with those of both parental strains. Interestingly, the symbiotic phenotype showed a marked cultivar-specific improvement with the cis-hybrid strains compared to both parental strains. These results provide a proof-of-principle for the feasibility of genome-wide replicon-based remodelling of bacterial strains for improved biotechnological applications in precision agriculture.
