Project description:This SuperSeries is composed of the following subset Series: GSE14055: Changes in polysome loading as a consequence of RHA downregulation GSE14056: Co-immunoprecipitation of RNAs with RNA helicase A (RHA) Refer to individual Series
Project description:The landscape of host circular and linear RNAs response to simian virus 40 infection unveils the key regulatory connections among diverse coding and noncoding RNAs
Project description:Given that RHA regulates translation by binding a PCE located at the 5’ UTR of the target transcripts a genome-wide screen was performed to identify mRNAs that bind RHA in vivo. The results from four experiments with samples obtained in four independent RNA immunoprecipitations identified 375 transcripts that co-immunoprecipitate with FLAG RHA. Since N-terminal FLAG tagged RHA specifically co-immunoprecipitates PCE-containing mRNAs HEK293 cells were transfected with a CMV-FLAG-RHA construct. Cytoplasmic lysates were immunoprecipitated with anti-FLAG beads. RNA was extracted from the immunoprecipitate and used to probe a human Agilent expression arrays. An immunoprecipitation with cells transfected with empty FLAG plasmid was used as negative control.
