Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Duck embryo fibroblasts infected with reovirus sequencing

PROVIDER: PRJNA1145885 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30211615_1.fastq.gz	Fastqsanger.gz
	SRR30211615_2.fastq.gz	Fastqsanger.gz
	SRR30211616_1.fastq.gz	Fastqsanger.gz
	SRR30211616_2.fastq.gz	Fastqsanger.gz
	SRR30211617_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Anas platyrhynchos

Project description:Duck embryo fibroblasts infected with duck plague virus sequencing

| PRJNA773993 | ENA

Project description:Coparative Transcriptiomic analysis of Duck Embryo Fibroblasts Infected Duck Tembusu Virus

| PRJNA624183 | ENA

Quantitative proteomic analysis of duck embryo fibroblasts infected with novel duck reovirus

Project description:The novel duck reovirus (NDRV) can cause hemorrhage and necrosis on the spleen of Pekin ducks, this disease has resulted in great economic losses to the duck industry. However, the molecular pathogenesis of NDRV remains poorly understood. In the current study, the quantitative proteomic analysis of NDRV-infected duck embryo fibroblasts was performed to explore the cellular protein changes in response to viral infection through iTRAQ coupled with the LC–MS/MS method. A total of 6,137 proteins were obtained in cell samples at 24 hours post infection. Of these, 179 differentially expressed proteins (DEPs) were identified (cutoff set to 1.5-fold change), including 89 upregulated and 90 downregulated proteins. Bioinformatic analysis showed that DEPs can be divided into the cellular component, molecular function, and biological process, they were mainly involved in the signal transduction, infectious diseases, cell growth and death, and the immune system. The subcellular localization of most proteins was cytoplasm. Importantly, the expression of signal transducer and activator of transcription 1 (STAT1) and various interferon-stimulated genes (ISGs) were upregulated after NDRV infection. The mRNA transcripts of some ISGs were consistent with proteomic data, showing an increased trend. Results of our study suggested that NDRV infection can elicit the strong expression changes of cellular proteins, and activate the expression of ISGs from the point of quantitative proteomic analysis. The study provides a new insight into the understanding of NDRV pathogenesis.

2021-09-07 | PXD020171 | Pride

Project description:Duck embryo fibroblasts cells infected with Newcastle disease virus (NDV)

| PRJNA953598 | ENA

Comparison of DEV infected CEF with control

Project description:Duck enteritis virus (DEV) is an important herpesvirus pathogen of waterfowl associated with an acute, highly contagious lethal disease. Using a deep sequencing approach on RNA from infected chicken embryo fibroblast (CEF) cultures, we determined the global changes in the microRNA (miRNA) expression profiles during DEV infection. In addition to the changes in the expression of a number of host miRNAs as a result of DEV infection, we identified several novel DEV-encoded miRNAs. Unlike most Mardivirus-encoded miRNAs, the majority of the DEV miRNAs were encoded within the unique long region of the viral genome. The precursors of DEV miR-D18 and miR-D19 overlapped with each other suggesting similarities to miRNA-offset RNAs, although only the DEV-miR-D18-3p was functional in reporter assays. Identification of these novel miRNAs will add to the growing list of virus-encoded miRNAs enabling the exploration of their roles in pathogenesis. Each microRNA is spotted on the array 6 times. We compared expression of duck enteritis virus (DEV)-infected chicken embryo fibroblasts (CEF) with CEF control.

2012-03-08 | E-GEOD-36258 | biostudies-arrayexpress

Comparative proteomic analysis revealed complex responses to classical/novel duck reovirus infections in Cairna moschata

Project description:Duck reovirus (DRV) is an typical aquatic bird pathogen belonging to the Orthoreovirus genus of the Reoviridae family. Reovirus causes a fatal disease resulting to huge economic losses to the duck industry. Although DRV has been identified and isolated long ago, the responses of Cairna moschata to classical/novel duck reovirus (CDRV/NDRV) infections are largely unknown. Using an integrated approach involving TMT labeling and LC-MS/MS analysis, proteomes of C. moschata liver cells under C/NDRV infections were analyzed. In total, 5571 proteins were identified, among which 5015 proteins were quantified. The differential expressed proteins (DEPs) between the control and infected liver cells displayed various biological functions and diverse subcellular localizations. Among the DEPs, most of the metabolism-related proteins were down-regulated, suggesting a decrease in the basal metabolisms under C/NDRV infections. Several important factors in the complement, coagulation and fibrinolytic systems were significantly up-regulated by C/NDRV infections, indicating that the serine protease-mediated innate immune system may play roles in the responses to C/NDRV infections. Moreover, a number of molecular chaperones were identified, and no significantly changes in their abundances were observed in the liver cells. Our data may give a comprehensive resource for investigating the regulation mechanism involved in the responses to C/NDRV infections in C. moschata.

2018-10-22 | PXD008623 | Pride

Genome-wide circRNA profiling reveals that circGAS2-2 are abundantly expressed and regulates myoblast proliferation in indigenous Chinese duck breed

Project description:The objective of this study was to identify candidate circular RNAs associated with duck muscle development. circRNA sequencing analysis was employed using female breast muscle samples embryo stage 13th day (E13) and embryo stage 19th day (E19). RNA-seq data and validation experiment in duck myoblast cells showed that circGAS2-2 significantly differential expressed between E13 and E19 group.

2023-03-20 | GSE152947 | GEO

Project description:Differential expression profile and in-silico functional analysis of long noncoding RNA and mRNA in duck embryo fibroblasts infected with avian reovirus

| PRJNA1044117 | ENA

Changes of miRNA expression profile after avian reovirus infection

Project description:We infected DF-1 cells with avian reovirus, and then used high-throughput sequencing to detect changes in miRNA expression profiles. This research provides a more comprehensive understanding of the interaction between viruses and host cells

2022-06-27 | GSE181193 | GEO

Transcriptomic profiles of gene expression of brain tissue in duck in response to Tembusu virus infection

Project description:We used high-throughput RNA-sequencing technology to reveal the transcriptome profile of DTMUV-infected duck brain tissue.

2020-04-29 | GSE142592 | GEO