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A cascade of RNA binding proteins controls the switch from proliferation to differentiation in an adult stem cell lineage [array]

ABSTRACT: A cascade of RNA binding proteins controls the switch from proliferation to differentiation in an adult stem cell lineage [array]

PROVIDER: PRJNA1146321 | ENA |

REPOSITORIES: ENA

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Project description:ABSTRACT: The mechanisms that regulate the switch from precursor cell proliferation to onset of differentiation in the adult stem cell lineages that maintain or repair many tissues in the body must be carefully regulated to assure production of sufficient progeny to maintain tissues yet prevent overproliferation that may lead to oncogenic progression. We are investigating the mechanisms regulating the switch from transit amplifying divisions to onset of differentiation in the Drosophila male germ line adult stem cell lineage, where spermatogonia undergo a limited number of mitotic divisions before switching to the spermatocyte program that sets up differentiation and meiosis. The number of transit amplifying spermatogonia divisions is set by accumulation of the Bag of marbles (Bam) protein to a critical threshold. Here we identify repression of Held Out Wings (how), homolog of mammalian Quaking, as the key target of Bam enabling the mitosis to differentiation switch. Knock down of how in germ cells was sufficient to allow spermatogonia mutant for bam or its partner bgcn to differentiate, while forced expression of nuclear-targeted How protein in otherwise wild-type spermatogonia resulted in continued proliferation of spermatogonia at the expense of differentiation. Our findings suggest that Bam targets how RNA for degradation by acting as an adapter to recruit the CCR4-NOT deadenylation complex via binding its subunit, Caf40. Our data support the model that the switch from proliferation to differentiation in this model adult stem cell lineage is regulated by a cascade of RNA-binding proteins. We used microarray to profile a time-course gene expression change from spermatogonia to spermatocytes for 48 hours in 8-hour intervals. We used the Bam-induced synchronous differentiation strategy we developed (PMID: 28522526). bam mutant testes are filled with developmentally arrested, overproliferating spermatogonia. By a pulse of Bam expression using 30 minutes of heat shock for the bam mutant flies bearing heat shock promoter driven Bam transgene, we induced synchronous differentiation of spermatogonia to spermatocytes. These cells stopped dividing and initiated the expression of early spermatocyte genes by 24 hours post-heat shock (PHS). By 48 PHS, they became young spermatocytes expressing hundreds of testis-specific genes. As a control, we used bam mutant flies without hs-Bam transgene but received the same 30 minutes of heat shock treatment.

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A cascade of RNA binding proteins controls the switch from proliferation to differentiation in an adult stem cell lineage [array]

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