Project description:PI3K-AKT signaling is essential for various cellular processes, including cell viability, proliferation, and metabolism. Here, we investigated the genome-wide changes induced by PI3K-AKTsignaling in primary granulosa cell cultures treated with control (DMSO) and PI3K-AKT pathway inhibitor LY294002.
Project description:Ovarian follicular luteal transformation is associated with induction of mitogen activated kinase (MAPK3/1) signaling. Here we investigated the genome wide changes induced by MAPK3/1 signaling in primary granulosa cell cultures treated with control (DMSO) and MAPK3/1 a.k.a ERK1/2 inhibitor PD98059.
Project description:Expression profiling of from DMSO and SP600125 treated glutamatergic neurons reveals JNK target genes that are transcriptionally regulated by JNK signaling. SP600125 is a known JNK signaling inhibitor and therefore, we used this to test whether the expression of JNK bound genes is affected upon inhibition of JNK kinase activity.
Project description:All-trans retinoic acid (ATRA) is important for sensitizing MM cells to carfilzomib (Cfz). To determine what signalling pathways are affected by ATRA in Cfz-treated MM cells, MM.1S MM cell line was pulsed with Cfz and then cultured with DMSO or 10µM ATRA for 12 h. Total RNAs of 2 x 106 MM.1S cells were extracted by RNeasy Mini Kit (Qiagen). 5-10 µg RNA samples were sent to Gene Expression and Genotyping Facility at Case Western Reserve University (Cleveland, OH) for genearray followed by data analysis. We use microarray data to determine differential expression of genes in Cfz-treated MM cells in culture with DMSO and ATRA.
Project description:Expression profiling of from DMSO and SP600125 treated glutamatergic neurons reveals JNK target genes that are transcriptionally regulated by JNK signaling. SP600125 is a known JNK signaling inhibitor and therefore, we used this to test whether the expression of JNK bound genes is affected upon inhibition of JNK kinase activity. Gene expression in the SP600125 treated neurons was compared to the DMSO treated neurons. a to e suffixes indicate biological replicates. Batch indicates different batches of the drug SP600125.
Project description:The aim of this study was to determine the role of ERβ in the response of mouse granulosa cells to PMSG (an FSH analog) by comparing the gene expression profiles of ERβ-het and ERβ-null animals treated with this compound.