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Naja nigricollis

ABSTRACT: Naja nigricollis

PROVIDER: PRJNA1147687 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR30501128_1.fastq.gz	Fastqsanger.gz
	SRR30501128_2.fastq.gz	Fastqsanger.gz

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Proteomic analysis of three medically important Nigerian Naja (Naja haje, Naja katiensis and Naja nigricollis) snake venoms

Project description:The venom proteomes of three medically important Nigerian Elapidae snakes Naja haje, Naja katiensis and Naja nigricollis was studied using Hydrophilic Interaction Liquid Chromatography (HILIC) coupled with LC-MS/MS analysis. Peptides/Proteins were identified and characterised using the SEQUEST and X!Tandem algorithms incorporated on to the Scaffold proteome software version 4.10.0. The Nigerian elapid species studied displayed about 70% similarity in composition of their venoms.

2021-03-12 | PXD024627 | Pride

North vs. South: Ecoregion-Specific Variations of Venom Toxin Profiles and Functional Activities in Nigerian Bitis arietans and Naja nigricollis

Project description:Snakebite envenoming is a major yet neglected tropical disease in sub-Saharan Africa, where antivenom efficacy is critically limited by intraspecific venom variation shaped by local'eco-logical pressures. Nigeria’s sharply contrasting Sudan Savanna (North) and Lowland Rainforest (South) provide an ideal natural system toinvestigate this variation, yet a comparative analysis of its medically important snakes has been lacking. We conducted an integrated proteomic and func-tional characterization of venoms from the puff adder (Bitis arietans) and black-necked spitting cobra (Naja nigricollis) collected in Kaduna (North) and Ibadan (South).Using high-resolution LC-MS/MS, SDS-PAGE, and biochemical assays (PLA₂, protease, fibrinogenolytic, haemolytic, and coagulation activities), we mapped region-specific venom compositions and corresponding toxic activities. Bitis arietans displayed striking ecoregion-driven divergence: southern populations were enriched in ser-ine proteases (27.54%), whereas northern venoms were dominated by lectins (25.4%) and distinct SVMP isoforms. Naja nigricollis showed a conserved PLA₂/3FTx backbone, yet southern venoms ex-hibited elevated SVMP III and LAAO abundance. These molecular differences manifested function-ally, with southern B. arietans showing significantly higher protease activity and southern N.nigricollis exhibiting enhanced PLA₂ activity. Principal component analysis suggested geography as a primary determinant of venom phenotype in both species. This work provides the first integrated proteomic and functional comparison of venoms from northern and southern Nigerian populations of Bitis arietans and Naja nigricollis. While based on a limited number of individuals, the observed differences reveal consistent regional trends that shouldbe interpreted as preliminary and hypoth-esis-generating rather than definitive population-level characteristics.

2026-06-01 | PXD073787 | Pride

An organotypic model of human skin simulates the dermonecrotic effects of Naja nigricollis envenoming

Project description:Snakebite envenoming is a neglected tropical disease that kills and maims hundreds of thousands annually. Naja nigricollis, the black-necked spitting cobra, has a cytotoxin-rich venom that is able to cause severe dermonecrosis and is not efficiently neutralized by current antivenoms. Here, we introduce an organotypic model of human skin to study the effects of exposure to N. nigricollis venom on human cells and compare it to the currently available in vivo mouse model. Histologically, the organotypic model simulates the severe necrotic lesions observed in mice. Proteomically, we show that among widespread global changes in protein abundance, many pathways involved in skin homeostasis and wound healing are specifically affected in both models. These results are the first to suggest that this organotypic model can simulate dermonecrosis caused by N. nigricollis venom and could thus be used to bridge the gap between in vitro and animal-based experiments for the study of the venom-induced cytotoxicity. This is an initial step towards replacing such animal-based experiments, which are associated with pain and tissue damage. The organotypic model may also find utility in evaluating the efficacy of new therapeutics against the severe and long-lasting consequences of snakebite envenoming in humans.

2023-01-11 | PXD033111 | Pride

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