Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1150979 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The microorganisms that colonized in or on hosts play significant roles in regulating host’s immunological fitness and bioenergy production, thus controls the host’s response to stress or foreign stimuli. Since radiation typically causes a pro-inflammatory and bioenergy expensive condition, we speculate that the radiation could influence gut microbial compositions, thereof the host-microbe bidirectional relationship. Pertinent model included exposing the young adult mice to total body irradiation (TBI) at doses of 9.5Gy and 11Gy, respectively. Descending colon contents (DCC) were collected from non-irradiated mice (baseline control), and irradiated mice euthanized on days 1, 3 and 9 post-TBI. DCC’s 16s ribosomal DNAs were screened for bacterial taxonomic classification and bacterial abundance profile informed metagenome-specific biofunctions. A second aliquot of same DCC was screened for untargeted metabolomics assay to find most perturbed metabolite-enriched networks. Dose-independent temporal delay since TBI emerged as the primary factor explaining the increased richness of bacterial community and the diversity of metabolite landscape. Among the mice exposed to 11Gy TBI, an increased abundance of Firmicutes, an anti-inflammatory and efficient energy harvesting bacteria accompanied an increment of pro-inflammatory Deferribacteres. Systems evaluation of the functional networks linked to DCC’s metagenome and metabolite, respectively, suggested a diverging trend between host and DCC microbes in regulating the network clusters, such as lipid metabolism, bioenergy synthesis, particularly at the later time points post-11Gy TBI. This analysis potentially suggested a disrupted symbiotic relationship between the host and microbes when the host became moribund.
2025-09-29 | GSE244571 | GEO
Project description:Colon contents of mice Raw sequence reads
| PRJNA1106081 | ENA
Project description:Eriocitrin, found in lemon fruit, has shown a wide range of biological properties. Herein, to evaluate the intestinal metabolic profile of eriocitrin in colon, the flavonoids in mice colon contents were identified by ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS), and a total of 136 flavonoids were found, including eriocitrin and its six metabolites (eriodictyol, homoeriodictyol, hesperetin, eriodictyol-3'-O-glucoside, hesperetin-7-O-glucoside and eriodictyol-7-O-(6''-O-galloyl) glucoside). Mice colon contents were used for 16S rDNA gene sequencing and gas chromatography-mass (GC-MS). Resultu showed that eriocitrin significantly alters the beta diversity of the gut microbiota, the probiotics such as Lachnospiraceae_UCG_006 were significantly enriched, and the production of butyrate, valerate and hexanoate in the colon pool of short-chain fatty acids (SCFAs) were significant increased. The spearman's association analysis performed some intestinal bacteria may be involved in the metabolism of eriocitrin. Collectively, our results preliminarily suggesting the metabolism of eriocitrin in the gut, demonstrate alterations of eriocitrin on gut microbiota, which warrants further investigation to determine its potential use in food and biomedical applications.
2022-11-30 | GSE218729 | GEO
Project description:16S rRNA gene sequencing analysis of the colon contents in mice
| PRJNA1224205 | ENA