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CUT&Tag Profiling Reveals Arid3a-Dependent Epigenetic Control in Vascular Smooth Muscle Cells

ABSTRACT: CUT&Tag Profiling Reveals Arid3a-Dependent Epigenetic Control in Vascular Smooth Muscle Cells

PROVIDER: PRJNA1152256 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30362718_1.fastq.gz	Fastqsanger.gz
	SRR30362718_2.fastq.gz	Fastqsanger.gz
	SRR30362719_1.fastq.gz	Fastqsanger.gz
	SRR30362719_2.fastq.gz	Fastqsanger.gz
	SRR30362720_1.fastq.gz	Fastqsanger.gz

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CUT&Tag Profiling Reveals Arid3a-Dependent Epigenetic Control in Vascular Smooth Muscle Cells

Project description:This study employs CUT&Tag technology to meticulously map the chromatin-binding landscape of the transcription factor Arid3a in Vascular Smooth Muscle Cells (VSMCs), thereby highlighting its crucial function in the intricate regulation of gene expression. The CUT&Tag assay illuminates Arid3a's presence at key promoter regions, exerting a profound impact on transcriptional modulation and suggesting the epigenetic governance of 260 genes. By integrating RNA-seq with sophisticated bioinformatic analyses, our research elucidates Arid3a's integral role in fundamental cellular activities, particularly in the dynamics of actin filaments, as well as its potential regulatory nexus with critical pathways, including cAMP signaling and calcium signaling. These insights unveil the intricate molecular choreography of Arid3a in the functioning of VSMCs and shed new light on its role in the development of abdominal aortic aneurysm (AAA).

2024-08-30 | GSE275619 | GEO

Cut&Tag profiling of NR1D1 in primary mouse vascular smooth muscle cells with or without vasostatin-2 stimulation

Project description:NR1D1 (also known as Rev-erbα) is a nuclear receptor involved in circadian rhythm and metabolic regulation. Its role in vascular biology is not fully understood. We performed CUT&Tag (Cleavage Under Targets and Tagmentation) to investigate the genome-wide binding profile of the transcription factor NR1D1 in primary vascular smooth muscle cells (VSMCs) isolated from mice. Two experimental conditions were included: cells treated with vasostatin-2 and untreated controls. The goal of this study was to identify potential transcriptional regulatory targets of NR1D1 under basal and vasostatin-2-stimulated conditions. CUT&Tag was carried out using a specific antibody against NR1D1, and sequencing was performed on the Illumina NovaSeq 6000 platform.

2025-09-01 | GSE295217 | GEO

Enhancer-associated LncRNA-ITGA2 promotes vascular smooth muscle remodeling by mediating ITGA2 promoter-enhancer interactions [CUT&TAG]

Project description:The proliferation and migration of VSMCs significantly contribute to vascular remodeling. Recent studies have suggested that enhancer-associated long noncoding RNAs (elncRNAs) play crucial roles in regulating gene expression and cell fate. However, the specific elncRNAs implicated in VSMC dysfunction and their regulatory mechanisms remain poorly understood. This study used multiomics profiling, including CUT&Tag, promoter capture Hi-C (PCHi-C), and microarray analysis, to identify LncRNA-ITGA2 as a novel elncRNA highly expressed in PDGF-induced proliferative human VSMCs. We used CUT&Tag technology to identify enhancer regions labeled by H3K4me1, and overlap lncRNA gene loci in LNCipedia database to identify enhancer-associated long noncoding RNAs (elncRNAs). By integrating the CUT&Tag data of H3K4me1 and H3K27ac, the dynamic regulatory network of enhancers was systematically analyzed, providing a key epigenetic basis for subsequent functional studies and target discovery.

2025-12-31 | GSE290462 | GEO

The megakaryocytic transcription factor ARID3A suppresses leukemia pathogenesis [CUT&RUN]

Project description:The purpose of this study was to decipher the molecular function of ARID3A. We leveraged gene expression (RNA-Seq) and chromatin profiling (ATAC-Seq and CUT&RUN) to evaluate gene expression changes upon restoring Arid3a expression in the context of the Gata1s mutation and miR-125b overexpression

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Epigenetic Regulation of VSMCs by Arid3a: Insights from RNA-seq Analyses

Project description:This study employs RNA-seq to investigate the transcriptional effects of Arid3a in Vascular Smooth Muscle Cells (VSMCs), revealing a comprehensive signature of gene expression. We identified 394 genes that were downregulated and 227 that were upregulated in response to Arid3a knockdown, shedding light on its regulatory network. The RNA-seq data highlighted the key role of Arid3a in controlling the dynamics of actin filaments, which has implications for processes such as cell adhesion and the cAMP signaling pathway.

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Project description:To map the chromatin binding sites for SMC1 in OE19 cells, we performed CUT&TAG.

2024-09-25 | E-MTAB-14090 | biostudies-arrayexpress

Cut&Tag profiling of NR1D1 in primary mouse vascular smooth muscle cells with or without vasostatin-2 stimulation

Project description:Cut&Tag profiling of NR1D1 in primary mouse vascular smooth muscle cells with or without vasostatin-2 stimulation

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CUT&TAG of OE19 cell line

Project description:To map the chromatin binding sites for various factors in OE19 cells, we performed CUT&TAG.

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