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Whole transcriptome analysis of control human fibroblasts, TGFβ-induced human fibroblasts, and fibroblasts co-cultured with normal lung decellularized scaffolds and fibroblasts co-cultured with bleomycin-induced lung decellularized scaffolds.

ABSTRACT: Whole transcriptome analysis of control human fibroblasts, TGFβ-induced human fibroblasts, and fibroblasts co-cultured with normal lung decellularized scaffolds and fibroblasts co-cultured with bleomycin-induced lung decellularized scaffolds.

PROVIDER: PRJNA1152263 | ENA |

REPOSITORIES: ENA

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Whole transcriptome analysis of control human fibroblasts, TGFβ-induced human fibroblasts, and fibroblasts co-cultured with normal lung decellularized scaffolds and fibroblasts co-cultured with bleomycin-induced lung decellularized scaffolds.

Project description:Fibrosis refers to the abnormal proliferation and excessive accumulation of fibrous tissue in an organ or tissue, typically caused by chronic injury or inflammation. Fibroblasts play a crucial role in the initiation and progression of fibrosis, with their excessive activation and overproduction of ECM being key mechanisms in fibrotic diseases. In this study, we constructed decellularized lung scaffolds from normal mice and bleomycin-induced lung decellularized scaffolds to analyze and compare the differential gene expression in control human fibroblasts, TGFβ-induced human fibroblasts, fibroblasts co-cultured with normal lung decellularized scaffolds, and fibroblasts co-cultured with bleomycin-induced lung decellularized scaffolds. This investigation aims to explore the impact of ECM on fibroblast activation and its underlying mechanisms.

2025-08-20 | GSE275625 | GEO

Matrisome properties of scaffolds direct fibroblasts in idiopathic pulmonary fibrosis

Project description:In this study we have focused on the biomechanical properties of scaffolds (decellularized lung tissue) derived from healthy individuals and IPF patients. The longitudinal cellular response of scaffold repopulation with healthy fibroblasts has been quantified using SILAC-MS. Increased scaffold density and stiffness along with differential expressions of proteins clearly separated and defined ECM proteins descriptive for IPF respective healthy scaffolds. Our study aim to understand the role of the ECM in the development of IPF. We have used proteomics to define intrinsic scaffold ECM proteins descriptive for healthy respective IPF scaffolds. To evaluate whether these mediators directs or rejects profibrotic responses fibroblasts repopulated on healthy and IPF derived scaffolds were cultured in heavy labeled medie (SILAC) to differentiate between preexisting scaffold proteins and newly synthesized proteins. The spatial distribution of unique ECM proteins characteristic for healthy fibroblasts on IPF scaffolds were verified with histological staining.

2019-08-27 | PXD012322 | Pride

Mapping of matrsisome production by alveolar epithelial cells cultured in decellularized human lung scaffolds

Project description:The alveolar epithelial cells are known producers of basement membrane components of the ECM, we hypothesize that they may also contribute substantially to remodeling of interstitial ECM in the alveolar compartment in chronic lung diseases such as chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis (IPF). Primary human alveolar type II epithelial cells (AECII) were isolated from human lungs without lung disease or with end stage COPD, using the surface marker HT2-280. Isolated cells were cryopreserved without prior expansion and then directly seeded into decellularized distal lung parenchyma from healthy human lungs. Cells were cultured for 13 days with addition of a profibrotic stimuli in the form of 2 ng/ml of TGF-ß1 at day 7, to a subgroup samples with cells derived from healthy lungs. AECII cells comes from 5 healthy cell donors and 3 with COPD, decellularized scaffolds were prepared from 5 different healthy lungs. The data set contains a total of 71 samples each representing a unique cell/scaffold/treatment combination at either day7 or day 13 of culture. For each sample there exists a matched proteomics sample from mass spectrometry analysis deposited to the ProteomeXchange Consortium via the PRIDE partner repository. The data indicate a substantial potential of alveolar epithelial cells to directly contribute to matrisome turnover and ECM remodeling in the lung.

2022-10-31 | GSE191279 | GEO

Mapping of matrsisome production by alveolar epithelial cells cultured in decellularized human lung scaffolds

Project description:Mapping of matrsisome production by alveolar epithelial cells cultured in decellularized human lung scaffolds

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mRNA expression of mid-old fibroblasts co-cultured with young and mid-old fibroblasts

Project description:Examination of the difference in mRNA expression profile between mid-old fibroblasts co-cultured with mid-old fibroblasts and mid-old fibroblasts co-cultured with young fibroblasts

2023-05-19 | GSE232425 | GEO

mRNA-seq of mouse lung vascular endothelial cells and mouse liver sinusoidal endothelial cells before and after their partial reprogramming into progenitor-like cells and recellularization of decellularized mouse lung scaffolds

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2020-06-23 | E-MTAB-8394 | biostudies-arrayexpress

Effect of mesenchymal stem cells on gene expression of fibroblasts when they were co-cultured

Project description:Our research and previous reports showed that fibroblasts could inhibit osteoblast differentiation of Mesenchymal stem cells (MSCs). Thus, RNA-seq was carried to analyze the transcriptome differences among fibroblasts cultured alone and fibroblasts co-cultured with MSCs to find detailed mechanisms about how fibroblasts had functions on MSCs. We then performed gene expression profiling analysis using data obtained from RNA-seq of fibroblasts cultured alone or co-cultured with MSCs.

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mRNA expression of mid-old fibroblasts co-cultured with young and mid-old fibroblasts

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Human Bone Marrow Stromal Cells cultured on 3D nanofiber scaffolds or 2D films

Project description:Microarray analysis was used to evaluate expression differences from a single donor human bone marrow stromal cells (hBMSCs) as a function of varied polymer-based tissue engineering scaffolds. These scaffolds include polycaprolactone (PCL) nanofibers (PCL_NF), films (PCL_SC), poly D,L-lactic acid (PDLLA) nanofibers (PDLLA_NF), films (PDLLA_SC), tissue culture polystyrene (TCPS) and TCPS with osteogenic supplements (TCPS_OS). The results revealed that scaffold structure was able to significantly affect gene expression, with nanofiber scaffolds inducing similar gene expression patterns to hBMCSs cultured with osteogenic media. A library of scaffolds prepared from polycaprolactone or poly D,L-lactic acid was sythesized and cultured with hBMSCs for 14 days with RNA extracted from cells on Day 1 and Day 14. Gene expression analysis was performed using BRB ArrayTools. SC = spun coat, BNF = big nanofiber, TCPS = tissue culture polystyrene, TCPS+OS = tissue culture polystyrene with osteogenic supplements. This data forms is part of a pending publication: Baker et al. Ontology Analysis of Global Gene Expression Differences of Human Bone Marrow Stromal Cells Cultured on 3D Scaffolds or 2D Films and is a subset of the 72 array data referenced in ( Kumar et al. The determination of stem cell fate by 3D scaffold structures through the control of cell shape, Biomaterials (2011) 32, 9188-9196.) The 72 array data set is submitted separately to GEO as GSE50743.

2013-11-01 | E-GEOD-50744 | biostudies-arrayexpress

Transcriptome analysis of primary airway epithelial cells co-cultured with human lung fibroblasts

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2022-07-01 | GSE205525 | GEO

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