Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

BEAM-T single cell sequencing of antigen-specific memory CD8+ T cell with age

ABSTRACT: BEAM-T single cell sequencing of antigen-specific memory CD8+ T cell with age

PROVIDER: PRJNA1152291 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30363600.fastq.gz	Fastqsanger.gz
	SRR30363601.fastq.gz	Fastqsanger.gz
	SRR30363602.fastq.gz	Fastqsanger.gz
	SRR30363603.fastq.gz	Fastqsanger.gz
	SRR30363604.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 96

Similar Datasets

BEAM-T single cell sequencing of antigen-specific memory CD8+ T cell with age

Project description:Aging trajectories of antigen-specific memory T cells are heterogenous and complex but incompletely understood. We use single-cell sequencing of EBV (Epstein Barr virus)- or VZV (Varicella Zoster virus)-antigen specific T cells of younger or older adults to analyze memory T cell heterogeneity in early and late life.

2025-06-01 | GSE275633 | GEO

Beam

Project description:Beam

| PRJEB60224 | ENA

ATAC-seq of antigen-specific memory CD8+ T cells

2025-06-01 | GSE275634 | GEO

Histone architecture of stem-cell memory T cells reveals progressive remodeling of epigenetic landscape in antigen-experienced CD8 T cells

Project description:We investigated the genomic landscape of histone modifications in antigen-experienced CD8+ T cells. Using a ChIP-Seq approach coupled with global gene expression profiling [GSE67825], we generated genome-wide histone H3 lysine 4 (H3K4me3) and H3 lysine 27 (H3K27me3) trimethylation maps in distinct subsets of CD8+ T cells - naïve, stem cell memory, central memory, and effector memory. To gain insight into how histone architecture is remodeled during the differentiation of activated T cells

2015-04-15 | E-GEOD-67881 | biostudies-arrayexpress

Metabolic quiescence of naïve-like memory T cells precedes and maintains antigen-specific T-cell memory

Project description:Metabolic quiescence of naïve-like memory T cells precedes and maintains antigen-specific T-cell memory

| PRJNA1268552 | ENA

Transcription profiling of mouse naive B cells vs. antigen-specific plasma, germinal center, and memory B cells after immunization with the T-dependent antigen, NP-CGG, in order to generate a transcriptional profile of self-renewal within the hematopoietic system

Project description:In order to better understand the factors that regulate B cell differentiation upon exposure to antigen, we compares global gene expression profiles from naive B cells with antigen-specific plasma, germinal center, and memory B cells after immunization with the T-dependent antigen, NP-CGG. The memory B cell-enriched transcripts were then compared with memory T cell-enriched and hematopoietic stem cell-enriched transcripts in order to generate a transcriptional profile of self-renewal within the hematopoietic system.

2007-12-15 | E-GEOD-4142 | biostudies-arrayexpress

Metabolic quiescence of naïve-like memory T cells precedes and maintains antigen-specific T-cell memory

Project description:Metabolic activity shapes cell fate but remains challenging to capture in vivo with high resolution. We performed longitudinal metabolic and phenotypic profiling of human antigen-specific CD8+ T cells after yellow-fever vaccination on the single-cell level. During the acute phase, T cells upregulated glycolysis to fuel anabolic needs of proliferation, but predominantly used oxidative phosphorylation for energy production, as assessed via protein translation rates. Central memory T cells were the most active subset, while effector cells underwent metabolic shut-down. In contrast, weakly differentiated naïve-like memory T cells showed minimal activity, relying solely on oxidative phosphorylation already during the acute phase. Reinforcing the link between cellular quiescence and longevity, naïve-like memory cells were preferentially maintained even 26 years post vaccination. This association between differentiation degree and metabolic activity was conserved after SARS-CoV-2 vaccination and in two murine infection models. Our study dissects the metabolic profile of antigen-specific T-cell responses ex vivo, highlighting quiescence as a key feature for long-term immunological memory formation in humans.

2025-11-17 | GSE298237 | GEO

Genome-wide analysis of gene methylation by effect of proton beam on breast cell line MCF-7

Project description:Genome wide DNA methylation profiling of estrogene receptor postive breast cancer cell line MCF-7, treating proton beam. The Illumina Infinium Human Methylation 450k Bead chip was used to obtain DNA methylation profiles across approximately 450,000 CpGs. This profiling indicates that proton beam induces epigenetic and cellular changes. Genomic DNA obtained from MCF-7 effected by proton beam

2015-06-19 | E-GEOD-70024 | biostudies-arrayexpress

T cell receptor signal strength and antigen affinity differentially regulate memory CD8+ T cell development

Project description:IL-2 inducible tyrosine kinase (Itk) is a Tec family non-receptor tyrosine kinase that is known to regulate T cell receptor signal strength (TcR) and T cell development and differentiation. TcR signal strength and antigen affinity are parameters known to regulate the development of CD8+ T cell memory. However, the intersection between TcR signal strength and antigen affinity on CD8+ memory T cell development remains unclear. Therefore, we compared the transcriptomes of WT and Itk-/- OT-1/Rag-/- CD8+ T cells at day 0 and day 7 following infection with Listeria monocytogenes expressing the SIINFEKL (N4) or SIITFEKL (T4) OVA peptide variant (different affinities for TcR) by RNA sequencing.

2020-09-30 | GSE137406 | GEO

Antigen-specific memory and naïve CD4+ T cells following secondary Chlamydia trachomatis infection

Project description:Memory antigen-specific CD4+ T cells against Chlamydia trachomatis are necessary for protection against secondary genital tract infection. While it is known that naïve antigen-specific CD4+ T cells can traffic to the genital tract in an antigen-specific manner, these T cells are not protective during primary infection. Here, we sought to compare the differences between memory and naïve antigen-specific CD4+ T cells in the same mouse following secondary infection using transgenic CD4+ T cells (NR1 T cells). Using RNA sequencing, we found that there were subtle but distinct differences between these two T cell populations. Naïve NR1 T cells significantly upregulated cell cycle genes and were more proliferative than memory NR1 T cells in the draining lymph node. In contrast, memory NR1 T cells were more activated than naïve NR1 T cells and were enriched in the genital tract. Together, our data provide insight into the differences between memory and naïve antigen-specific CD4+ T cells during C. trachomatis infection.

2020-05-01 | GSE146265 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data