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Scanning and Mining of High Fecundity Genes by Oxford Nanopore Technologies (ONT) in Sheep (Ovis aries) Pituitary

ABSTRACT: Scanning and Mining of High Fecundity Genes by Oxford Nanopore Technologies (ONT) in Sheep (Ovis aries) Pituitary

PROVIDER: PRJNA1152572 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30404909_1.fastq.gz	Fastqsanger.gz
	SRR30404910_1.fastq.gz	Fastqsanger.gz
	SRR30404911_1.fastq.gz	Fastqsanger.gz
	SRR30404912_1.fastq.gz	Fastqsanger.gz
	SRR30404913_1.fastq.gz	Fastqsanger.gz

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Scanning and Mining of High Fecundity Genes by Oxford Nanopore Technologies (ONT) in Sheep (Ovis aries) Pituitary

Project description:The pituitary gland is an important endocrine organ that regulates estrus and reproduction in sheep mainly through hormone synthesis and secretion. In present study, Small-tailed Han sheep (high-reproduction group, HP group), and Wadi sheep (lower-reproduction group, LP group), were used as the experiment materials, and the differential expressed genes (DEGs) were scanned and mined by Oxford Nanopore Technologies (ONT) in pituitary. A total of 7123 DEGs were found, including 3551 genes that were upregulated and 3572 genes that were downregulated in HP group. Go and KEGG related to pituitary function and reproduction were enriched, including reproductive processes, responses to stimuli, and synapses. mTOR signaling pathway, PI3K-Akt signaling pathway, cAMP signaling pathway, ERK1/2 signaling pathways and MAPK signaling pathways. The DEGs detected in this study were involved in the development of tissues and organs and the secretion of hormones in the endocrine system. These findings suggest that these genes might be related to growth, development and the reproduction regulation in sheep, which could provide a scientific basis for elucidating the genetic mechanisms of high reproduction in sheep.

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Identification of reproduction-related genes in the hypothalamus of the sheep (Ovis aries) using the ONT full-length transcriptome sequencing technology

Project description:the hypothalamus tissues of high-reproduction small-tailed Han sheep and low-reproduction Wadi sheep were collected, and full-length transcriptome sequencing by Oxford Nanopore Technologies (ONT) was performed to explore the key functional genes associated with sheep fecundity. The differentially expressed genes (DEGs) were screened and enriched using DESeq2 software through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).

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