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ZNF574 is a Quality Control Factor for Defective Ribosome Biogenesis Intermediates [RNA-Seq]

ABSTRACT: ZNF574 is a Quality Control Factor for Defective Ribosome Biogenesis Intermediates [RNA-Seq]

PROVIDER: PRJNA1154244 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30476759.fastq.gz	Fastqsanger.gz
	SRR30476760.fastq.gz	Fastqsanger.gz
	SRR30476761.fastq.gz	Fastqsanger.gz
	SRR30476762.fastq.gz	Fastqsanger.gz

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ZNF574 is a Quality Control Factor for Defective Ribosome Biogenesis Intermediates [CRISPRi_FLOW]

Project description:ZNF574 is a Quality Control Factor for Defective Ribosome Biogenesis Intermediates [CRISPRi_FLOW]

| PRJNA1154238 | ENA

ZNF574 is a Quality Control Factor for Defective Ribosome Biogenesis Intermediates [CRISPRi_growth]

Project description:ZNF574 is a Quality Control Factor for Defective Ribosome Biogenesis Intermediates [CRISPRi_growth]

| PRJNA1154243 | ENA

ZNF574 is a Quality Control Factor for Defective Ribosome Biogenesis Intermediates [CRISPRi_FLOW]

Project description:Eukaryotic ribosome assembly is an intricate process that involves four ribosomal RNAs, 80 ribosomal proteins, and over 200 biogenesis factors that take part in numerous interdependent steps. The complexity and essentiality of this process creates opportunities for deleterious mutations to occur, accumulate, and impact downstream cellular processes. “Dead-end” ribosome intermediates that result from biogenesis errors are rapidly degraded, affirming the existence of quality control pathway(s) that monitor ribosome assembly. However, the factors that differentiate between on-path and dead-end intermediates are unknown. We engineered a system to perturb ribosome assembly in human cells and discovered that faulty ribosomes are degraded via the ubiquitin proteasome system. We identified ZNF574 as a key component of a novel quality control pathway, which we term the Ribosome Assembly Surveillance Pathway (RASP). In an animal model, loss of ZNF574 leads to developmental defects, further emphasizing the importance of RASP in organismal health.

2025-05-30 | GSE275990 | GEO

ZNF574 is a Quality Control Factor for Defective Ribosome Biogenesis Intermediates [CRISPRi_growth]

2025-05-30 | GSE275991 | GEO

ZNF574 is a Quality Control Factor Targeting Defective Ribosome Biogenesis Intermediates

Project description:The eukaryotic ribosome is a complex molecular machine responsible for the translation of mRNA to protein. Faulty ribosome production leads to decreased protein production, increased cellular stress, and often cell death. How defective ribosomes are detected and cleared is poorly understood. We modeled ribosome biogenesis failure in human cells and performed CRISPRi screens to identify quality control factors that cope with trapped biogenesis intermediates. We identified ZNF574 as the top hit. ZNF574 contains Cys2-His2 (C2H2) zinc finger binding domains that are known to interact with both DNA and RNA, providing a potential mechanism for recognizing and engaging defective ribosomes. ZNF574 localizes in the nucleoplasm and cellular speckles, similar to late ribosome biogenesis factors like eIF6 and NMD3. We confirmed that depletion of ZNF574 impedes the degradation of faulty ribosomes. We performed immunoprecipitation followed by mass spectrometric analysis. Our immunoprecipitation of GFP-tagged ZNF574 followed by mass spectrometry analysis of interacting proteins revealed strong enrichment for ribosomal proteins from the large subunit. Together, our data show that ZNF574 functions as a quality control factor that targets defective large subunits.

2025-04-28 | PXD051645 | Pride

ZNF574 is a Quality Control Factor for Defective Ribosome Biogenesis Intermediates [RNA-Seq]

2025-05-30 | GSE275992 | GEO

Ubiquitination of Stalled Ribosome Triggers Ribosome-associated Quality Control

Project description:Ubiquitination of Stalled Ribosome Triggers Ribosome-associated Quality Control

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Post-transcriptional regulation of Ribosome Biogenesis

Project description:Post-transcriptional regulation of Ribosome Biogenesis

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GIGYF2 and 4EHP Inhibit Translation Initiation of Defective Messenger RNAs to Assist Ribosome-Associated Quality Control

Project description:GIGYF2 and 4EHP Inhibit Translation Initiation of Defective Messenger RNAs to Assist Ribosome-Associated Quality Control

| PRJNA643292 | ENA

The E3 SUMO ligase Mms21 controls ribosome biogenesis

Project description:The budding yeast E3 SUMO ligase Mms21, a component of the Smc5-6 complex, regulates sister chromatid cohesion, DNA replication, and DNA repair. We identify a role for Mms21 in ribosome biogenesis. The mms21RINGD mutant exhibits reduced rRNA production, nuclear accumulation of 60S and 40S ribosomal proteins, and elevated Gcn4 translation. Genes involved in ribosome biogenesis and translation are down-regulated in the mms21RINGD mutant. Examining gene expression profile of mms21RINGD mutant compared to wild-type by RNA Seq using Ilumina sequencing

2015-06-13 | E-GEOD-69826 | biostudies-arrayexpress

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