Umbilical Cord Blood miRNA Profiling: Links to Maternal Metabolism and Neonatal Traits
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1156183 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR30534206.fastq.gz | Fastqsanger.gz | |||
| SRR30534207.fastq.gz | Fastqsanger.gz | |||
| SRR30534208.fastq.gz | Fastqsanger.gz | |||
| SRR30534209.fastq.gz | Fastqsanger.gz | |||
| SRR30534210.fastq.gz | Fastqsanger.gz |
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Project description:We performed miRNA profiling of umbilical cord plasma (UCP) to investigate the association of circulating neonatal miRNAs with maternal metabolic parameters and neonatal anthropometric, metabolic, and inflammatory characteristics in healthy pregnancies. We collected data and UCP samples from 16 pregnancies, in equal parts with normal weight and overweight mothers, and male and female neonates. We performed next-generation miRNA sequencing and analysed metabolic and inflammatory parameters in UCP. Our results revealed that the majority of UCP miRNAs are sensitive to maternal and neonatal characteristics, particularly to maternal BMI, Our study highlights the susceptibility of UCP miRNAs to maternal metabolism, demonstrates their association with neonatal metabolic and inflammatory traits, and underscores the potential role of circulating umbilical cord blood miRNAs in fetal metabolism and development.
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Project description:MicroRNAs (miRNAs) are regulators of mRNA translation and play crucial roles in various physiological and pathological processes. In this study, we profiled miRNAs in umbilical cord plasma (UCP) to explore the association of neonatal circulating miRNAs with maternal metabolic parameters and neonatal anthropometric, metabolic and inflammatory characteristics in healthy pregnancies. Data and UCP samples were collected from 16 pregnancies, equally divided between normal-weight and overweight mothers and between male and female newborns. Using next-generation sequencing, we identified and quantified miRNAs in UCP, alongside the analysis of metabolic and inflammatory parameters. Our results revealed that the majority of UCP miRNAs are sensitive to maternal and neonatal characteristics, particularly maternal body mass index, gestational weight gain, placental weight, UCP leptin, UCP C-reactive protein and UCP insulin levels. Notably, we identified a strong association between the placenta-derived chromosome 19 microRNA cluster (C19MC) and placental weight, gestational weight gain, UCP insulin and neonatal weight. Likewise, the pregnancy-specific chromosome 14 microRNA cluster (C14MC) was associated with maternal body mass index and UCP leptin. Our study highlights the sensitivity of UCP miRNAs to maternal metabolic conditions, demonstrates their association with neonatal metabolic and inflammatory traits, and underscores the potential role of circulating cord blood miRNAs in fetal metabolism and development. KEY POINTS: MicroRNAs (miRNAs) are regulatory RNA molecules that modulate protein expression. They are present in all body fluids and umbilical cord plasma and are affected by metabolic changes. Pregnancy is a state of metabolic change in the mother, and maternal metabolism affects fetal development. We found that the composition of umbilical cord blood miRNAs is associated with maternal and neonatal metabolism. Pregnancy-specific groups of miRNAs showed particular patterns, with miRNAs encoded by a region of chromosome 14 associated with maternal body mass index and with miRNAs encoded by a specific region of chromosome 19 associated with umbilical cord plasma insulin. MicroRNAs represent a separate dimension through which maternal metabolism can influence fetal development.
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Project description:Umbilical cord blood could serve as useful source of blood markers enabling more efficient and reliable prenatal and neonatal diagnostics. MicroRNAs (miRNAs) are ubiquitous in body fluids where they were used for detecting and monitoring various physiological and pathological conditions. In this descriptive study, we aimed to identify changes in miRNA expression profiles associated with basic maternal somatic and epidemiological characteristics.
2019-03-28 | GSE128943 | GEO
Project description:Analysis of umbilical cord tissue in newborns of type 1 diabetic mothers at gene expression level. The hypothesis tested in the present study was that intrauterine diabetic milieu may effect of fetal umbilical cord gene expression, and via umbilical cord, the alterations may be produced in other fetal tissues as well. Results provide an information of the differentially expressed genes and enriched pathways, such as the dowregulated genes on the pathway on blood vessel development in umbilical cords from diabetic pregnancies.
2016-01-01 | GSE51546 | GEO
Project description:Protein glycosylation is an essential post-translational modification boosting proteomic diversity. The major groups of human plasma glycoproteins are immunoglobulins (Igs) and acute phase proteins (APPs). Changes in plasma Igs and APPs levels and their glycosylation have been previously associated with inflammatory diseases, several types of cancer, pregnancy, or aging. In this study, we present a novel approach to investigate the neonatal and maternal plasma at the end of pregnancy using a combination of targeted quantitative proteomics and total plasma N-glycome profiling. We present a new SRM-based multiplex assay to quantify 19 APPs and 7 Igs. We applied this assay to 98 samples of umbilical cord plasma and corresponding maternal plasma samples and observed significantly lower levels of all analyzed proteins in neonates, apart from the glycoprotein alpha-2-macroglobulin. Unlike previous methods, our SRM-MS assay allows us to quantify dozens of proteins simultaneously and to distinguish protein variants. We compared maternal and fetal total plasma-released N-glycomes in the same set of samples. We quantified 66 individual glycans, which were used to calculate 73 glycosylation traits. In neonates, the major N-glycans were di-antennary complex-type with core fucosylation, and the production of higher, branched, sialylated N-glycans was limited.
2025-09-15 | PXD068295 | panorama
Project description:Analysis of umbilical cord tissue in newborns of type 1 diabetic mothers at gene expression level. The hypothesis tested in the present study was that intrauterine diabetic milieu may effect of fetal umbilical cord gene expression, and via umbilical cord, the alterations may be produced in other fetal tissues as well. Results provide an information of the differentially expressed genes and enriched pathways, such as the dowregulated genes on the pathway on blood vessel development in umbilical cords from diabetic pregnancies. Umbilical cord tissue was collected after elective ceasarean section and was immediately flash frozen in liquid nitrogen and stored at -80C until total RNA extraction from the whole tissue sample. Six cords exposed to maternal diabetes (DM) and six control cords (C) from healthy pregnancies were analyzed.
2016-07-03 | E-GEOD-51546 | biostudies-arrayexpress
Project description:Neonatal health is dependent on early risk stratification, diagnosis, and timely management of many potentially devastating conditions. Preterm infants are at increased risk of prematurity-related complications, including: early-onset sepsis, chronic lung disease, intraventricular hemorrhage, necrotizing enterocolitis, and neurodevelopmental impairment.Many of these conditions are poorly predicted in real-time by clinical data, including currently available diagnostic testing. Thus, biomarkers have been sought to aid early and targeted treatment and prognosis for these conditions. Umbilical cord blood may represent a novel source of molecular signatures that provides a window into the state of the fetus at birth. Umbilical cord blood inflammatory markers have been studied as diagnostic indicators of early-onset sepsis. Specific cord blood cytokines have been studied as predictors or correlates of retinopathy of prematurity, atopic disease, infantile hemangioma, placental histopathology, and more. However, few of these cord blood biomarkers have been translated into diagnostic tools in clinical practice. Longitudinal profiling of postnatal proteomic changes has provided insights into the development of the immune system over the first weeks to months of life. While proteomic profiling of cord blood has demonstrated immunologic differences between preterm and term infants, prior research has lacked inclusion of preterm infants across the continuum of gestational age and consideration of key perinatal characteristics such as the route of delivery, preeclampsia, intraamniotic infection, and neonatal sepsis that are likely to affect protein expression. In this study, we have comprehensively characterized the cord blood proteome from infants born between 25 to 42 weeks using MS to provide a benchmark of normative cord blood proteomic profile and examine proteome differences across the developmental range of gestational ages.
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Project description:ObjectiveNo validated biomarker at birth exists to predict which newborns will develop severe hyperbilirubinemia. This study's primary aim was to build and validate a prediction model for severe hyperbilirubinemia using umbilical cord blood bilirubins (CBB) and risk factors at birth in neonates at risk for maternal-fetal blood group incompatibility. This study's secondary aim was to compare the accuracy of CBB to the direct antigen titer.MethodsInclusion criteria for this prospective cohort study included: ≥35 weeks gestational age, mother with blood type O and/or Rh negative or positive antibody screen, and <24 hours of age. The primary outcome was severe hyperbilirubinemia, defined as phototherapy during the initial hospital stay. Secondary outcomes were a total serum bilirubin concentration >95th and >75th percentile during the initial hospital stay. The predictive performance and accuracy of the two tests (CBB and direct antigen titer) for each outcome was assessed using area under a receiver-operating characteristic curve (AUC), sensitivity, and specificity.ResultsWhen compared to neonates who did not receive phototherapy (n = 463), neonates who received phototherapy (n = 36) had a greater mean CBB ± standard deviation (2.5 ± 0.7 vs. 1.6 ± 0.4 mg/dL, p<0.001). For every 0.3 mg/dL increase in CBB, a neonate was 3.20 (95% confidence interval, 2.31-4.45), 2.10 (1.63-2.70), and 3.12 (2.44-3.99) times more likely to receive phototherapy or have a total serum bilirubin concentration >95th and >75th percentile, respectively. The AUC ± standard error (95% confidence interval) for CBB for phototherapy and a total serum bilirubin concentration >95th and >75th percentile was 0.89 ± 0.03 (0.82-0.95), 0.81 ± 0.04 (0.73-0.90), and 0.84 ± 0.02 (0.80-0.89), respectively. However, the AUC for gestational age and maternal Asian race for these outcomes was only 0.55 ± 0.05 (0.45-0.66), 0.66 ± 0.05 (0.56-0.76), and 0.57 ± 0.04 (0.05-0.64), respectively. When the CBB was combined with gestational age and maternal Asian race, the AUC for a total serum bilirubin concentration >95th percentile improved to 0.87 ± 0.03 (0.81-0.92) (p = 0.034 vs. the model with CBB only and p<0.001 vs. the model with clinical risk factors only). In a sub-group of subjects (n = 189), the AUC for the direct antigen titer for phototherapy was 0.64 ± 0.06 (0.52-0.77) with a 52% sensitivity and 77% specificity. In contrast, a CBB cut-point of 1.85 mg/dL was 92% sensitive and 70% specific for phototherapy with an AUC of 0.87 ± 0.04 (0.80-0.95).ConclusionCBB, in combination with gestational age and maternal race, may be a useful, non-invasive test to predict shortly after birth which neonates will develop severe hyperbilirubinemia.
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