Project description:We divided the e male C57BL/6 mice into two groups: a non-treatment group and an IMQ-induced psoriasiform dermatitis group. The dorsal root ganglia of the mice were extracted, enzymatically digested, and then enriched using Percoll. Subsequently, single-cell RNA sequencing was performed.
Project description:We divided the male C57BL/6 mice into two groups: a non-treatment group and an IMQ-induced psoriasiform dermatitis group. We isolated the C3-C5 ganglia from male mice, then preserved them in RNA stabilization solution. RNA was extracted using Trizol, followed by transcriptome sequencing.
Project description:We use dorsal root ganglion tissues of WT SD rats, or imiquimod(IMQ)-induced psoraisis-like rat model treated with or without epidural injection of 1% lidocaine. We isolated total RNA for RNA-sequencing.
Project description:To investigate the mechanism underpinning the effect of pregnancy on psoriasis, we conducted bulk RNA sequencing on the dorsal lesional skin of imiquimod (IMQ)-induced psoriatic dermatitis mice under pregnant and non-pregnant conditions, as well as on blank controls. This analysis revealed the critical role of crosstalk between dermal fibroblasts and macrophages.
Project description:To investigate the mechanism underlying the effect of pregnancy on psoriasis, we performed single-cell RNA sequencing (scRNA-seq) on the dorsal lesional skin of imiquimod (IMQ)-induced psoriatic dermatitis mice in pregnant versus non-pregnant states. This analysis uncovered the critical role of crosstalk between dermal fibroblasts and macrophages.