Project description:We divided the e male C57BL/6 mice into two groups: a non-treatment group and an IMQ-induced psoriasiform dermatitis group. The dorsal root ganglia of the mice were extracted, enzymatically digested, and then enriched using Percoll. Subsequently, single-cell RNA sequencing was performed.
Project description:We divided the male C57BL/6 mice into two groups: a non-treatment group and an IMQ-induced psoriasiform dermatitis group. We isolated the C3-C5 ganglia from male mice, then preserved them in RNA stabilization solution. RNA was extracted using Trizol, followed by transcriptome sequencing.
Project description:We use dorsal root ganglion tissues of WT SD rats, or imiquimod(IMQ)-induced psoraisis-like rat model treated with or without epidural injection of 1% lidocaine. We isolated total RNA for RNA-sequencing.
Project description:In order to establish a consensus catalog of dorsal rott ganglion cell types, we used comprehensive transcriptome analysis of single cells for unsupervised identification and molecular classification of sensory neurons independent of any a priori knowledge of sensory subtypes. RNA-Seq was performed on 799 dissociated single cells dissected from the mouse lumbar dorsal root ganglion distributed over a total of nine 96-well plates
Project description:miR-146a acts as a negative feedback regulator of inflammation. To investigate the role of miR-146a in psoriasis psoriasiform skin inflammation was indeuced in Mir-146a-/- and wild type mice (C57BL6J) by topical applciation of imiquimod (IMQ)-cream (Aldara). Gene expression profiling (Affymetrix) was used to identify transcriptomic changes associated with psoriasis-like skin inflammation in wild type vs. miR-146a -/-mice. A daily topical dose of 31.25 mg of Aldara cream (5% IMQ) was applied on the right ear of miR-146a -/- and C57BL/6 mice on three consecutive days to induce psorisis-like skin inflammation. Mice were sacrificed at day 4. Ear flaps were collected for total RNA extraction and hybridization on Affymatrix GeneTitan plate format Gene ST 2.1 (mouse).