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ABSTRACT: Structural basis for nucleolin recognition of MYC promoter G-quadruplex

PROVIDER: PRJNA1156828 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30557318_1.fastq.gz	Fastqsanger.gz
	SRR30557318_2.fastq.gz	Fastqsanger.gz
	SRR30557319_1.fastq.gz	Fastqsanger.gz
	SRR30557319_2.fastq.gz	Fastqsanger.gz
	SRR30557320_1.fastq.gz	Fastqsanger.gz

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Structural basis for nucleolin recognition of MYC promoter G-quadruplex

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G-Quadruplex structures are stable and detectable in human genomic DNA.

Project description:The G-quadruplex is an alternative DNA structural motif that is considered to be functionally important in the mammalian genome. Herein, we address the hypothesis that G-quadruplex structures can exist within double-stranded genomic DNA using a G-quadruplex-specific probe. An engineered antibody is employed to enrich for DNA containing G-quadruplex structures, followed by deep sequencing to detect and map G-quadruplexes at high resolution in genomic DNA from human breast adenocarcinoma cells. Our high sensitivity structure-based pull-down strategy enables the isolation of genomic DNA fragments bearing a single as well as multiple G-quadruplex structures. Stable G-quadruplex structures are found in sub-telomeres, gene bodies and gene regulatory regions. For a sample of identified target genes, we show that G-quadruplex stabilizing ligands can modulate transcription. These results confirm the existence of G-quadruplex structures and their persistence in human genomic DNA. Four independent libraries have been enriched in DNA G-quadruplex structures using a G-quadruplex-specific probe. One genomic input library was sequenced as control. Deep-sequencing of these libraries allowed the mapping of G-quadruplexes on the genome.

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