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Mesenchymal SLMAP coordinates with MST3 to govern gut elongation during development

ABSTRACT: Mesenchymal SLMAP coordinates with MST3 to govern gut elongation during development

PROVIDER: PRJNA1158222 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR30589130_1.fastq.gz	Fastqsanger.gz
	SRR30589130_2.fastq.gz	Fastqsanger.gz
	SRR30589131_1.fastq.gz	Fastqsanger.gz
	SRR30589131_2.fastq.gz	Fastqsanger.gz

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Mesenchymal SLMAP coordinates with MST3 to govern gut elongation during development

Project description:Developing gut in mice undergoes rapid elongation during late embryogenesis, yet significantly slows down after birth. Precise regulatory mechanism of this dynamic morphogenetic process remains unknown. Utilizing scRNA-seq analysis, we show that YAP activity in Cxcl13high intestinal fibroblasts is the major molecular contributor to gut elongation. To determine how mesenchymal YAP activity is controlled, we identified canonical Sarcolemma membrane-associated protein (SLMAP) as its critical regulator during embryonic gut morphogenesis. Deleting Slmap in gut mesenchyme impairs YAP activity, leading to short gut and a significant decrease in intestinal epithelial cell proliferation. Mechanistically, Slmap activates YAP by directly regulating Mst3 kinase. Physiologically, Mst3 levels prominently increase over the developmental time, reaching their peak on postnatal day P14, when gut elongation in mice slows down. Depleting Mst3 in mesenchyme results in increased gut length at P14 accompanied by enhanced YAP activity. Importantly, short gut phenotype in SlmapcKO mice is partially compensated by concomitant deletion of mesenchymal Mst3. Taken together, our findings demonstrate that Slmap interacts with Mst3 kinase to dynamically regulate mesenchymal YAP activity that governs gut elongation across its embryonic and postnatal development.

2025-06-16 | GSE276611 | GEO

Mesenchymal SLMAP coordinates with MST3 to govern gut elongation during development [RNAseq_TL]

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Mesenchymal SLMAP coordinates with MST3 to govern gut elongation during development [RNAseq_TL]

Project description:Mesenchymal SLMAP coordinates with MST3 to govern gut elongation during development [RNAseq_TL]

| PRJNA1158481 | ENA

Mesenchymal SLMAP coordinates with MST3 to govern gut elongation during development (scRNA-Seq)

Project description:Developing gut in mice undergoes rapid elongation during late embryogenesis, yet significantly slows down after birth. Precise regulatory mechanism of this dynamic morphogenetic process remains unknown. Utilizing single-cell RNA-sequencing analysis, we show that YAP activity in Cxcl13high intestinal fibroblasts is the major molecular contributor to gut elongation. To determine how mesenchymal YAP activity is controlled, we identified canonical Sarcolemma membrane-associated protein (SLMAP) as its critical regulator during embryonic gut morphogenesis. Deleting Slmap in gut mesenchyme impairs YAP activity, leading to short gut and a significant decrease in intestinal epithelial cell proliferation. Mechanistically, SLMAP activates YAP by directly regulating MST3 kinase. Physiologically, MST3 levels prominently increase over the developmental time, reaching their peak on postnatal day P14, when gut elongation in mice slows down. Depleting Mst3 in mesenchyme results in increased gut length at P14 accompanied by enhanced YAP activity. Importantly, short gut phenotype in mesenchyme-specific Slmap mutant mice is partially compensated by concomitant deletion of mesenchymal Mst3. Taken together, our findings demonstrate that SLMAP interacts with MST3 kinase to dynamically regulate mesenchymal YAP activity that governs dynamic gut elongation across its embryonic and postnatal development.

2025-06-16 | GSE277051 | GEO

Mesenchymal SLMAP coordinates with MST3 to govern gut elongation during development (scRNA-Seq)

Project description:Mesenchymal SLMAP coordinates with MST3 to govern gut elongation during development (scRNA-Seq)

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ROS-induced cytosolic release of PGAM5 accelerates cancer progression via MST3-YAP axis

Project description:Aberrant mtROS release in response to mitochondrial stress are considered to be a major novel feature of neoplastic transformation. However, the molecular mechanisms by which mtROS contribute to CRC development have not been fully elucidated. In this study, we showed that upon sensing the mtROS signal, the mitochondrially localized phosphatase PGAM5 undergoes cleavage in the mitochondrial transmembrane domain, and then the Cleaved PGAM5 is released into the cytoplasm. Subsequently, Cleaved PGAM5 binds to the kinase MST3 in the cytoplasm to promote MST3 dephosphorylation, resulting in a decrease in MST3 activity. Importantly, MST3 inactivation failed to regulate YAP phosphorylation, leading to YAP translocation into the nucleus, consequently promoting CRC proliferation and metastasis. Collectively, our findings identified the PGAM5-MST3-YAP axis as an important molecular mechanism through which mtROS promotes CRC development.

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