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Alteration of global transcription by saccharin in Escherichia coli K12

ABSTRACT: Alteration of global transcription by saccharin in Escherichia coli K12

PROVIDER: PRJNA1158806 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30606311_1.fastq.gz	Fastqsanger.gz
	SRR30606311_2.fastq.gz	Fastqsanger.gz
	SRR30606312_1.fastq.gz	Fastqsanger.gz
	SRR30606312_2.fastq.gz	Fastqsanger.gz
	SRR30606313_1.fastq.gz	Fastqsanger.gz

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Alteration of global transcription by saccharin in Escherichia coli K12

Project description:We perform differential RNA-seq comparing a treatment with 1.4% saccharin to a mock treatment in E. coli K12 in order to describe the alterations in global transcription produced by this artificial sweetener. Three biological replicates of saturated E. coli K12 cultures were diluted 1/100 in 25 mL LB medium (in duplicate, to obtain 3 treated cultures and 3 mock controls). The cultures were grown (37 ºC, 180 rpm) to approximately OD600 0.3 and they were treated with a final concentration of 1.4% saccharin or a water control. The cultures were further incubated until reacing OD600 0.8. At this point, cells were harvested, treated with RNAlate for preservation of total RNA and stored at -80 C. After that, total RNA was extracted from each sample. As a result, 305 genes appeared upregulated by the saccharin treatment, whereas 419 were downregulated.

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Alteration of global transcription by saccharin in Acinetobacter baumannii AB5075

Project description:We perform RNA-seq comparing a treatment with 1% saccharin to a mock treatment in A. baumannii AB5075 in order to describe the alterations in global transcription produced by this artificial sweetener. A. baumannii AB5075 cultures were grown in LB medium in the presence of 1% saccharin or a water control (3 biological replicates per condition) until reaching mid-exponential phase. At this point, cells were harvested, treated with RNAlate for preservation of total RNA and stored at -80 C. After that, total RNA was extracted from each sample. As a result, 165 genes appeared upregulated in the presence of saccharin, whereas 215 were downregulated. In highlights, the gene functional groups showing the strongest transcriptional alterations were those related to pili and frimbriae biogenesis, involved in biofilms formation, adhesion and twitching motility.

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