Project description:We report a mass spectrometry-based proteomics approach to advance the study of the feline proteome. We analyzed immortalized feline cells, various tissues and plasma using data-dependent acquisition (DDA) on a Q Exactive HF mass spectrometer to develop a Felis Catus PeptideAtlas from 157 acquired runs. Data were analyzed using the Trans-Proteomic-Pipeline. We report in the first PeptideAtlas of the domestic cat the identification of 242,844 unique peptides representing 8,972 feline proteins or 45.6% of the predicted UniProtKB Felis catus proteome based on 19,655 unique genes and their representative protein sequences.
Project description:This work presents, fort the first time, the comparative study of in vitro behavior, cytogenetics and molecular analysis by LC-MS/MS of two Echinococcus granulosus s.s/G1 metacestodes found in two naturally unrelated Felis catus FIV-free
