Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Three-dimensional genome structures of single mammalian sperm

ABSTRACT: Three-dimensional genome structures of single mammalian sperm

PROVIDER: PRJNA1160276 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30644800_1.fastq.gz	Fastqsanger.gz
	SRR30644800_2.fastq.gz	Fastqsanger.gz
	SRR30644900_1.fastq.gz	Fastqsanger.gz
	SRR30644900_2.fastq.gz	Fastqsanger.gz
	SRR30645000_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 5956

Similar Datasets

Three-dimensional genome structures of single mammalian sperm [GAM]

Project description:Three-dimensional genome structures of single mammalian sperm [GAM]

| PRJNA1160225 | ENA

Three-dimensional genome structures of single mammalian sperm [GAM]

Project description:The three-dimensional (3D) organization of chromosomes is crucial for packaging a large mammalian genome into a confined nucleus and ensuring proper nuclear functions in somatic cells. However, the packaging of the much more condensed sperm genome is fundamentally different and not as well understood. In this study, we resolved the 3D whole-genome structures of a single mammalian sperm cell using an enhanced chromosome conformation capture assay. The reconstructed genome structures accurately delineate the species-specific nuclear morphologies for both human and mouse sperm. We discovered that sperm genomes are divided into chromosomal territories and A/B compartments, similarly as somatic cells. However, neither human nor mouse sperm chromosomes contain topologically associating domains or chromatin loops. These results suggest that the fine-scale chromosomal organization of mammalian sperm fundamentally differs from that of somatic cells.

2025-03-09 | GSE277016 | GEO

Three-dimensional genome structures of single mammalian sperm [scHi-C]

Project description:Three-dimensional genome structures of single mammalian sperm [scHi-C]

| PRJNA1160215 | ENA

Three-dimensional genome structures of single mammalian sperm [scHi-C]

2025-03-09 | GSE276999 | GEO

Three-dimensional genome structures of single mammalian sperm [bulk Hi-C]

2025-03-09 | GSE277018 | GEO

Three-dimensional genome structures of single mammalian sperm [bulk Hi-C]

Project description:Three-dimensional genome structures of single mammalian sperm [bulk Hi-C]

| PRJNA1160231 | ENA

Single-cell three dimensional genome structures of rice egg, sperm, and unicellular zygote

Project description:Chromatin conformation capture assays, such as 3C and Hi-C, allow for studies of three-dimensional (3D) genome structures in bulk samples through proximity ligation of DNA. However, the difference between cells can only be observed by single-cell measurements that avoid ensemble averaging3, 4,5. To study 3D chromatin organization and dynamics before and after fertilization in flowering plants, we analyzed the 3D genomes of rice egg, sperm, and unicellular zygote as well as shoot cells. We show that chromatin architectures of rice egg and sperm are comparable to that of somatic cells and are reorganized after fertilization in unicellular zygote. The rice single cell 3D genomes display specific features of chromosome compartments and configuration of telomeres/centromeres compared to those shown in mammalian single cells. Active and silent chromatin domains gather to form multiple foci in the nuclear space. Notably, the 3D genomes of egg, unicellular zygote, and shoot cells contain a compact silent center (CSC), which is absent in sperm. CSC is dynamically reorganized after fertilization and is likely to be involved in gene regulation related to zygotic genome activation (ZGA). Our results reveal specific 3D genome features of plant gametes and unicellular zygote and provide a spatial chromatin basis for ZGA and gene expression in plant.

2019-05-26 | GSE123109 | GEO

Single-cell three dimensional genome structures of rice egg, sperm, and unicellular zygote

Project description:Single-cell three dimensional genome structures of rice egg, sperm, and unicellular zygote

| PRJNA507566 | ENA

Three-dimensional genome structures of single mammalian sperm

Project description:This SuperSeries is composed of the SubSeries listed below.

2025-03-08 | GSE277040 | GEO

Cryo-electron microscopy unravels microtubule inner protein networks reinforcing mammalian sperm flagella

Project description:To find and fuse with the egg, mammalian sperm must complete an arduous voyage through the female reproductive tract. This odyssey is powered by the sperm tail, a specialized motile cilium. Mammalian sperm tails are reinforced at the molecular scale with sperm-specific microtubule inner proteins (sperm-MIPs), but the identities of these sperm-MIPs are unknown. Here, we report high-resolution cryo-electron microscopy structures of bovine sperm doublet microtubules (DMTs), allowing us to identify many sperm-MIPs. We also resolve structures of singlet MTs in the endpiece, revealing MIPs shared between singlet and doublet MTs. We demonstrate that at least two sperm-MIPs bind and stabilize MTs in vitro. Our structures shed light on ciliary diversity across cell types and provide structural frameworks for understanding molecular underpinnings of male infertility

2023-06-20 | PXD035941 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data