Project description:NCIH2170 is a lung cancer cell line harboring a large amount of extrachromosomal DNA (ecDNA) with high cell-to-cell variation. We performed 10X sc-multiome sequencing on this cell line to study the pattern of gene expression and chromatin accessibility with the existence of ecDNA.
Project description:As the light-sensing part of the visual system, the retina is comprised of five classes of neurons, including photoreceptors, horizontal, amacrine, bipolar, and retinal ganglion cells, along with several non-neuronal cell types such as Muller glia. These major cell classes can be further classified into hundreds of distinct cell subtypes. The development of the retina is under tight temporal control where multipotent progenitor cells differentiate into specific mature cell types in a sequential, but overlapping, order. Additionally, the developmental process is under tight spatial control, with cells at the central retina developing earlier than cells at the periphery. To provide a comprehensive view of the human fetal retina at the molecular level and investigate transcriptional regulatory mechanisms controlling the differentiation process, we profiled more than 300,000 single nuclei of the human fetal retina from 12 donors spanning post conception week 10 and 23 with Multiome RNA-seq and ATAC-seq.
Project description:We performed single-nucleus ATAC-seq (snATAC-seq) to profile chromatin accessibility across eight brain regions from male C57BL/6J mice and 10x Multiome to jointly profile chromatin accessibility and gene expression in the same regions from female C57BL/6J mice, at 2, 9, and 18 months of age. Nuclei were isolated from frozen dissected brain tissue, tagmented with barcoded Tn5 transposase, and sequenced using either combinatorial barcoding (male samples) or 10x Multiome (female samples). Our analysis reveals region- and age-specific chromatin accessibility changes, highlighting regulatory programs associated with brain aging in distinct cell types.
Project description:We sequenced 2 heart samples from human fetal donors and detected the differential expressed mRNAs and lncRNAs. The network of significant differential expressed transcripts could be associated to developmental program. examination mRNA and lncRNA expression in heart tissues
