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Altered translation elongation contributes to key hallmarks of aging in killifish brain (tRNA-Seq)

ABSTRACT: Altered translation elongation contributes to key hallmarks of aging in killifish brain (tRNA-Seq)

PROVIDER: PRJNA1162539 | ENA |

REPOSITORIES: ENA

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Altered translation elongation contributes to key hallmarks of aging in killifish brain (tRNA-Seq)

Project description:Aging is a major risk factor for neurodegeneration and is characterized by diverse cellular and molecular hallmarks. To understand their origin, we studied the effects of aging on the transcriptome, translatome, and proteome in the brain of short-lived killifish. We identified a cascade of events in which aberrant translation pausing led to altered abundance of proteins independently of transcriptional regulation. In particular, aging caused increased ribosome stalling and widespread depletion of proteins enriched in basic amino acids. These findings uncover a potential vulnerable point in the aging brain's biology – the biogenesis of basic DNA- and RNA-binding proteins. This vulnerability may represent a unifying principle that connects various aging hallmarks, encompassing genome integrity, proteostasis and the biosynthesis of macromolecules.

2025-07-31 | GSE277509 | GEO

Altered translation elongation contributes to key hallmarks of aging in killifish brain

Project description:Altered translation elongation contributes to key hallmarks of aging in killifish brain

| PRJNA972265 | ENA

Altered translation elongation contributes to key hallmarks of aging in killifish brain

2025-07-31 | GSE232465 | GEO

Altered translation elongation contributes to key hallmarks of aging in killifish brain (RNA-Seq)

2025-07-31 | GSE277507 | GEO

Altered translation elongation contributes to key hallmarks of aging in killifish brain (Ribo-Seq)

2025-07-31 | GSE277506 | GEO

Altered translation elongation contributes to key hallmarks of aging in killifish brain (RNA-Seq)

Project description:Altered translation elongation contributes to key hallmarks of aging in killifish brain (RNA-Seq)

| PRJNA1162541 | ENA

Altered translation elongation contributes to key hallmarks of aging in killifish brain (Ribo-Seq)

Project description:Altered translation elongation contributes to key hallmarks of aging in killifish brain (Ribo-Seq)

| PRJNA1162540 | ENA

RNA Seq of the ventricular region from young and aged killifish

Project description:In order to understand molecular basis of cardiac ageing in killifish, we have performed RNA Seq on the ventricular regions of young and aged killifish. Experiment was performed in biological triplicates.

2019-03-22 | E-MTAB-7582 | biostudies-arrayexpress

Identification of nascent peptides of peptidyl-tRNA dissociated from ribosome in the early elongation stage in Escherichia coli pthts cells

Project description:Accurate translation of mRNAs into functional proteins is a fundamental process in all living organisms. In bacteria, in the early stage of translation elongation, peptidyl-tRNAs (pep-tRNAs) with short nascent chains frequently dissociate from the ribosome (pep-tRNA drop-off). The dissociated pep-tRNAs are deacylated and recycled by peptidyl-tRNA hydrolase (PTH), which is an essential enzyme in bacteria. Here, we establish a highly sensitive method for direct profiling of pep-tRNAs using RNA isolation method and mass spectrometry. We isolated each tRNA species with peptide from Escherichia coli pthts cells using reciprocal circulating chromatography and precisely analyzed their nascent peptides. As a result, we successfully detected 703 peptides consisted of 402 cognate peptides and 301 non-cognate peptides with single amino-acid substitution. Detailed analysis of individual pep-tRNAs revealed that most of the substitutions in the miscoded peptides take place at the C-terminal drop-off site. We further examined this observation using a reporter construct and found that the non-cognate pep-tRNAs produced by mistranslation rarely participate in the next round of elongation but dissociate from the ribosome, suggesting that pep-tRNA drop-off is an active mechanism by which the ribosome rejects miscoded pep-tRNAs in the early elongation, thereby contributing to quality control of protein synthesis after peptide bond formation.

2023-05-18 | PXD030808 | Pride

Identification of nascent peptides of peptidyl-tRNA dissociated from ribosome in the early elongation stage in Escherichia coli pthts cells

2023-05-18 | PXD030805 | Pride