Project description:Histologically normal human breast FFPE specimens were profiled by NanoString nCounter to study parity-conditioned basal/progenitor, Hedgehog, and vitamin D receptor-associated biology in normal breast tissue. This workbook contains the Breast Cancer 360 (BC360) arm of the study and is paired with a companion workbook for the other NanoString panel. Each of the 74 analyzed specimens contributes one GEO sample to this platform-specific workbook and one GEO sample to the companion panel workbook.
Project description:We analyzed a cohort of 220 early breast cancer patients with ER-positive and/or progesterone receptor (PR)-positive, HER2-negative tumors.
Project description:Histologically normal breast lobules were obtained from non-Hispanic Black women (NHBW) and non-Hispanic White women (NHWW) (n=10 per group) matched by age, BMI, and parity. RNA extracted from FFPE sections was profiled using the NanoString Breast Cancer 360 CodeSet to characterize transcriptional programs associated with group differences in benign breast epithelium.
Project description:Baseline lobule-enriched FFPE RNA from benign breast biopsies of women aged 45–55 was profiled using NanoString nCounter PanCancer IO 360 and Breast Cancer 360 panels. Sample identifiers were anonymized (ARLI###) for public release; the original internal block codes are retained only in a private key held by the authors. Gene expression was related to the subsequent pace of age-related lobular involution across the menopausal transition.
Project description:Individualized outcome prediction classifiers were successfully constructed through expression profiling of a total of 758 (BC360 9.15.2023) and 750 (IO360 9.15.2023) genes in 20 breast cancer (BC) cases. Differential expression on Oncologic (BC30) and Immunooncogenic IO360) profiling was performed using nCounter NanoString technology.