Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

An AT-hook transcription factor promotes transcription of histone, spliced-leader, and piRNA clusters [mRNA-Seq]

ABSTRACT: An AT-hook transcription factor promotes transcription of histone, spliced-leader, and piRNA clusters [mRNA-Seq]

PROVIDER: PRJNA1163267 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30734006_1.fastq.gz	Fastqsanger.gz
	SRR30734006_2.fastq.gz	Fastqsanger.gz
	SRR30734007_1.fastq.gz	Fastqsanger.gz
	SRR30734007_2.fastq.gz	Fastqsanger.gz
	SRR30734008_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

An AT-hook transcription factor promotes transcription of histone, spliced-leader, and piRNA clusters

Project description:In all three domains of life, genes with related functions can be enriched in specific genomic regions, known as the gene clusters. In eukaryotes, histone, piRNA, and rDNA (ribosomal DNA) clusters are among the most notable clusters which play fundamental roles in chromatin formation, genome integrity, and translation, respectively. These clusters have traditionally been thought to be regulated by distinct transcriptional mechanisms. In this study, using C. elegans as a model system we identify ATTF-6, a member of the AT-hook family, as a key factor for the expression of histone, piRNA, and 5S rDNA-SL1 (spliced leader 1) clusters. ATTF-6 forms distinct nuclear foci at both piRNA and 5S rDNA-SL1 clusters. Loss of ATTF-6 leads to a depletion of histone mRNAs and SL1 transcripts. Additionally, we demonstrate that ATTF-6 is required for the recruitment of USTC (Upstream Sequence Transcription Complex) at piRNA clusters, which is necessary for piRNA production. Collectively, our findings underscore the essential role of an AT-hook transcription factor in promoting the expression of fundamental gene clusters.

2025-01-29 | PXD055895 | Pride

An AT-hook transcription factor promotes transcription of histone, spliced-leader, and piRNA clusters [smllRNA-seq]

Project description:In all three domains of life, genes with related functions can be organized into specific genomic regions known as gene clusters. In eukaryotes, histone, piRNA, and rDNA (ribosomal DNA) clusters are among the most notable clusters which play fundamental roles in chromatin formation, genome integrity, and translation, respectively. These clusters have long been thought to be regulated by distinct transcriptional mechanisms. In this study, using C. elegans as a model system we identify ATTF-6, a member of the AT-hook family, as a key factor for the expression of histone, piRNA, and 5S rDNA-SL1 (spliced leader 1) clusters. ATTF-6 forms distinct nuclear foci at both piRNA and 5S rDNA-SL1 clusters. Loss of ATTF-6 leads to a depletion of histone mRNAs and SL1 transcripts. Additionally, we demonstrate that ATTF-6 is required for the recruitment of USTC (Upstream Sequence Transcription Complex) to piRNA clusters, which is necessary for piRNA production. Collectively, our findings reveal a unifying role for an AT-hook transcription factor in promoting the expression of fundamental gene clusters.

2025-01-28 | GSE277643 | GEO

An AT-hook transcription factor promotes transcription of histone, spliced-leader, and piRNA clusters [mRNA-Seq]

2025-01-28 | GSE277642 | GEO

An AT-hook transcription factor promotes transcription of histone, spliced-leader, and piRNA clusters [ChIP-Seq]

2025-01-28 | GSE277641 | GEO

An AT-hook transcription factor promotes transcription of histone, spliced-leader, and piRNA clusters [smllRNA-seq]

Project description:An AT-hook transcription factor promotes transcription of histone, spliced-leader, and piRNA clusters [smllRNA-seq]

| PRJNA1163269 | ENA

An AT-hook transcription factor promotes transcription of histone, spliced-leader, and piRNA clusters [ChIP-Seq]

Project description:An AT-hook transcription factor promotes transcription of histone, spliced-leader, and piRNA clusters [ChIP-Seq]

| PRJNA1163259 | ENA

An AT-hook transcription factor promotes transcription of histone, spliced-leader, and piRNA clusters.

Project description:In all three domains of life, genes with related functions can be organized into specific genomic regions known as gene clusters. In eukaryotes, histone, piRNA (Piwi-interacting RNA), and rDNA (ribosomal DNA) clusters are among the most notable clusters which play fundamental roles in chromatin formation, genome integrity, and translation, respectively. These clusters have long been thought to be regulated by distinct transcriptional mechanisms. In this study, using Caenorhabditis elegans as a model system we identify ATTF-6, a member of the AT-hook family, as a key factor for the expression of histone, piRNA, and 5S rDNA-SL1 (spliced leader 1) clusters. ATTF-6 is essential for C. elegans viability. It forms distinct nuclear foci at both piRNA and 5S rDNA-SL1 clusters. Loss of ATTF-6 leads to a depletion of histone mRNAs, SL1 transcripts, and piRNAs. Additionally, we demonstrate that ATTF-6 is required for the recruitment of USTC (Upstream Sequence Transcription Complex) to piRNA clusters, which is necessary for piRNA production. Collectively, our findings reveal a unifying role for an AT-hook transcription factor in promoting the expression of fundamental gene clusters.

| S-EPMC11822377 | biostudies-literature

Genome-wide map of spliced-leader-led trans-splice sites in Oikopleura dioica

Project description:Polycistronic mRNAs transcribed from operons are resolved via the trans-splicing of a spliced leader (SL) RNA. The SL is also frequently trans-spliced to monocistronic transcripts. Using a modified cap analysis of gene expression (CAGE) protocol we mapped sites of SL trans-splicing genome-wide in the marine chordate Oikopleura dioica and find evidence for proposed functions of SL-trans-splicing. A recent hypothesis postulates that operons facilitate recovery from growth arrested states in metazoans. We examined the expression dynamics of operons across the life-cycle of the animal and during growth arrest recovery. We show that operons do not facilitate recovery from growth arrest in O. dioica. We find that operons are enriched in the germline and that trans-spliced transcripts are predominantly maternal., Interestingly, there is a TOP-like motif in the SL sequence, and trans-splicing in TOP mRNAs, indicating that trans-spliced mRNAs are targets for nutrient-dependent translational control in O. dioica. Total RNA from a number of stages across development were pooled and used in a modified DeepCAGE protocol. A custom designed spliced-leader primer (using the SL exon) was used in the 2nd strand synthesis step.

2015-01-26 | E-GEOD-50849 | biostudies-arrayexpress

Genome-wide map of spliced-leader-led trans-splice sites in Oikopleura dioica

2015-01-26 | GSE50849 | GEO

Functional characterisation of nematode spliced leader 1 trans-splicing factors SUT-1, SNA-2, SNA-3 and SNR-2 by immunoprecipitation and LC-MS/MS.

Project description:Spliced leader trans-splicing is an essential RNA processing step that is required for the formation of mRNA in many eukaryotes, including C. elegans. However, the role of factors involved in this reaction is not well known. Here we further characterise the function of SNA-2, SNA-3, SUT-1 and SNR-2 involved in spliced leader 1 trans-splicing through identification of interacting proteins by immunoprecipitation followed by LC-MS/MS and label-free quantification.

2024-04-23 | PXD040352 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data