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Hippophae rhamnoides

ABSTRACT: Transcriptomes of developing fruits of sea buckthorn varieties

PROVIDER: PRJNA1163394 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30830326_1.fastq.gz	Fastqsanger.gz
	SRR30830326_2.fastq.gz	Fastqsanger.gz
	SRR30830327_1.fastq.gz	Fastqsanger.gz
	SRR30830327_2.fastq.gz	Fastqsanger.gz
	SRR30830328_1.fastq.gz	Fastqsanger.gz

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Gene expression profiles modulated by lipophilic sea buckthorn (Hippophae rhamnoides L.) extract in BT-549 triple-negative breast cancer cells

Project description:Considering that dysregulation of cholesterol levels has been established as one of the hallmarks of cancer, moreover ferroptosis, autophagy, tumor cell, immune cell stemness, and the cellular DNA damage response are regulated through cholesterol metabolism, while recent research studies have shown that dietary supplementation with sea buckthorn reduced total cholesterol only in people with abnormal lipid metabolism, the aim of this study was to identify, for the first time, the main signaling pathways, biological processes, cellular functions, and upstream regulators modulated by lipophilic sea buckthorn extract (LSBE) in BT-549 TNBC cell line.

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TMT-based Quantitative Proteomics of Developing Sea Buckthorn (Hippophae rhamnoides) Berries Reveals Candidate Proteins Related to Lipid Metabolism

Project description:Sea buckthorn (Hippophae rhamnoides L.) is an economically important shrub or small tree indigenous to Asia and Europe. The most well-recognized medicinal and nutraceutical products derive from the berry oil, which is rich in essential fatty acids, monounsaturated palmitoleic acid (16:1 ω-7), linoleic (18:2 ω-6) and alpha-linolenic (18:3 ω-3) acids. In this study, tandem mass tags (TMT) MS/MS were used to investigate protein profiles of lipid metabolism in sea buckthorn berries harvested at 30, 50 and 70 days after flowering. A total of 8626 proteins were detected and 6170 of these were quantified. Further analyses of the proteins and their pathways revealed initiation of fatty acid accumulation throughout the whole berry development. Activity of most of key enzymes, which are related to biosynthesis pathways of triacylglycerol and fatty acids, had peaked at 50 days; but triacylglycerol synthesis through the PDAT (phospholipid: diacylglycerol acyltransferase) catalytic pathway of occurred mostly at the early stage of berry development. Protein expression patterns related to lipid accumulation were also verified at the transcript level using quantitative real-time polymerase chain reaction. The proteome profiles determined here will improve our understanding of the process of lipid accumulation and the dynamic changes in metabolic pathways during sea buckthorn berry development.

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Multi-omic data reveals the role of the antioxidant network in the quality of growing sea buckthorn fruit

Project description:Fruits of sea buckthorn have abundant antioxidants and attractive colors. They provide excellent materials to study the relationships among fruit color, antioxidant and fruit quality

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