Gallus gallus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA116379 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Microarrays experiments were performed in order to assess which genes are differentially expressed between the embryonic chicken sinus horns at HH14. Three, technical, dye swapped comparisons between Left and Right sinus horns. In total six arrays were used in this study
2013-05-01 | E-GEOD-16433 | biostudies-arrayexpress
Project description:Microarrays experiments were performed in order to assess which genes are differentially expressed between the embryonic chicken sinus horns at HH14.
2013-05-01 | GSE16433 | GEO
Project description:We report the transcriptomes of 10 different chicken (Gallus gallus) cell/tissue types. The goal of this project was to determine similarities and differences between different cell/tissue types, with respect to protein coding genes, lncRNA, isoform counts, and differential gene expression. We provide raw data and bigWig files for UCSC visualization. The findings described here will be useful towards a complete annotation of chicken tissue and cellular transcriptomes.
2021-04-26 | GSE166257 | GEO
Project description:Asymmetrical gonadal development is an intriguing phenomenon observed in the majority of female birds. In chickens, the left gonad of female embryos develops into a functional ovary, while the right gonad undergoes degeneration during embryogenesis. This sexually dimorphic trait is primarily induced by the spatial differential expression of the PITX2 gene. However, a comprehensive understanding of the transcriptional profile of the developing gonads during asymmetric development is still lacking. To elucidate the molecular mechanism of asymmetric gonadal development in chickens, we compared the transcriptomes between left and right gonads of female chickens using bulk- and single cell (sc) -RNA sequencing (RNA-seq) approaches. Our bulk RNA-seq analysis of the female chicken gonads at E5 (HH26), E6.5 (HH30), E8 (HH34), and E9.5 (HH36) revealed significant differential gene expression between the left and right female chicken gonads, particularly in signaling pathways, cell cycle, and metabolic processes. Moreover, scRNA-seq analysis revealed that coelomic epithelial, interstitial, and pre-granulosa cells of the left gonads share a highly proliferative status, contributing to the asymmetric gonadal cell proliferation, which may be regulated by the TGFβ signaling pathway. Our findings demonstrate that dynamic cell-type-specific transcriptional profiles during embryogenesis play a vital role in the asymmetric gonadal development of female chickens.
2025-09-26 | GSE278924 | GEO
Project description:Asymmetrical gonadal development is an intriguing phenomenon observed in the majority of female birds. In chickens, the left gonad of female embryos develops into a functional ovary, while the right gonad undergoes degeneration during embryogenesis. This sexually dimorphic trait is primarily induced by the spatial differential expression of the PITX2 gene. However, a comprehensive understanding of the transcriptional profile of the developing gonads during asymmetric development is still lacking. To elucidate the molecular mechanism of asymmetric gonadal development in chickens, we compared the transcriptomes between left and right gonads of female chickens using bulk- and single cell (sc) -RNA sequencing (RNA-seq) approaches. Our bulk RNA-seq analysis of the female chicken gonads at E5 (HH26), E6.5 (HH30), E8 (HH34), and E9.5 (HH36) revealed significant differential gene expression between the left and right female chicken gonads, particularly in signaling pathways, cell cycle, and metabolic processes. Moreover, scRNA-seq analysis revealed that coelomic epithelial, interstitial, and pre-granulosa cells of the left gonads share a highly proliferative status, contributing to the asymmetric gonadal cell proliferation, which may be regulated by the TGFβ signaling pathway. Our findings demonstrate that dynamic cell-type-specific transcriptional profiles during embryogenesis play a vital role in the asymmetric gonadal development of female chickens.
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Project description:Gene expression profiling between chicken tissues
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Project description:HH stage 21 chicken dorsal mesentery is laser microdissected into four morphological compartments, and subject to global expression analyses on Affymetrix Chicken 3' microarray Goal is to identify genes expressed in each of left epitelium, left mesenchyme, right mesenchyme, right epithelium; genes on the left may be direct or indirect transcriptional targets of Pitx2.
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