Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CUT&RUN of the aging mouse brain reveals origins of inflammation in the white matter

ABSTRACT: CUT&RUN of the aging mouse brain reveals origins of inflammation in the white matter

PROVIDER: PRJNA1164126 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30767473_1.fastq.gz	Fastqsanger.gz
	SRR30767473_2.fastq.gz	Fastqsanger.gz
	SRR30767474_1.fastq.gz	Fastqsanger.gz
	SRR30767474_2.fastq.gz	Fastqsanger.gz
	SRR30767475_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 52

Similar Datasets

Spatial transcriptomics and CUT&RUN of the aging mouse brain reveal origins of inflammation in the white matter

Project description:Spatial transcriptomics and CUT&RUN of the aging mouse brain reveal origins of inflammation in the white matter

| PRJNA1197659 | ENA

White matter aging drives microglial diversity

Project description:Aging results in both grey and white matter degeneration, but the specific microglial responses are unknown. Using single-cell RNA sequencing from white and grey matter separately, we identified white matter associated microglia (WAM), which share parts of the disease-associated microglia (DAM) gene signature and are characterized by the activation of genes implicated in phagocytic activity and lipid metabolism. WAM depend on triggering receptor expressed on myeloid cells 2 (TREM2) signaling and are aging dependent. In the aged brain, WAM form independently of apolipoprotein E (APOE), which is in contrast to mouse models of Alzheimer’s disease, in which microglia with WAM gene signature are generated prematurely and in an APOE-dependent pathway similar to DAM. Within the white matter, microglia frequently cluster in nodules, where they are engaged in clearing degenerated myelin. Thus, WAM may represent a potentially protective response required to clear degenerated myelin accumulating during white matter aging and disease.

2021-02-18 | GSE166548 | GEO

White matter aging drives microglial diversity

Project description:White matter aging drives microglial diversity

2021-02-08 | GSE166304 | GEO

CD8+ T cells induce interferon-responsive oligodendrocytes during white matter aging

Project description:A hallmark of nervous system aging is a decline of white matter volume and function, but the underlying mechanisms leading to white matter pathology are unknown. Here, we found age-related alterations of oligodendrocytes with a reduction of total oligodendrocyte density in the aging murine white matter. Using single-cell RNA sequencing, we identify interferon-responsive oligodendrocytes, which localize in proximity of CD8+ T cells in the aging white matter. Absence of functional lymphocytes decreased oligodendrocyte reactivity and rescued oligodendrocyte loss, while T-cell checkpoint inhibition worsened the aging effect. In addition, we identified a subpopulation of immune cell dependent interferon-responsive microglia in the aging white matter, and co-culture experiments revealed that interferon- activated microglia triggered oligodendrocytes cell death. In summary, we provide evidence that T cells induced interferon-responsive oligodendrocytes and microglia are important modifiers of white matter aging.

2022-09-13 | GSE202579 | GEO

CUT&RUN of the aging mouse brain reveals origins of inflammation in the white matter

Project description:To systematically understand age-induced molecular changes, we performed spatial transcriptomics of young, middle-aged, and old mouse brains and identified seven transcriptionally distinct regions. All regions exhibited age-associated upregulation of inflammatory mRNAs and downregulation of mRNAs related to synaptic function. Notably, aging white matter fiber tracts showed the most prominent changes with pronounced effects in females. The inflammatory signatures indicated major ongoing events: microglia activation, astrogliosis, complement activation, and myeloid cell infiltration. Immunofluorescence and quantitative MRI analyses confirmed physical interaction of activated microglia with fiber tracts and concomitant reduction of myelin in old mice. In silico analyses identified potential transcription factors influencing these changes. Our study provides a resourceful dataset of spatially resolved transcriptomic features in the naturally aging murine brain encompassing three age groups and both sexes. The results link previous disjointed findings and provide a comprehensive overview of brain aging identifying fiber tracts as a focal point of inflammation.

2025-01-02 | GSE277768 | GEO

White matter aging drives microglial diversity

Project description:White matter aging drives microglial diversity

| PRJNA700408 | ENA

White matter aging drives microglial diversity

Project description:White matter aging drives microglial diversity

| PRJNA701252 | ENA

Regional vulnerability of brain white matter in VWM

Project description:Vanishing white matter (VWM) is a leukodystrophy that primarily manifests in young children. In this disease, the brain white matter is differentially affected in a predictable pattern with telencephalic brain areas being more severely affected, while others remain allegedly completely spared. Using high-resolution mass spectrometry-based proteomics, we investigated the proteome patterns of the severely affected white matter in the frontal lobe and normal appearing pons in VWM and control cases to identify molecular bases underlying regional vulnerability. By comparing VWM patients to controls, we identified disease-specific proteome patterns. We showed substantial pathogenic changes in both the frontal white matter and pons at the protein level. Side-by-side comparison of brain region-specific proteome patterns further revealed regional differences. We found that different cell types are affected in the VWM frontal white matter than in the pons. Gene ontology and pathway analyses identified involvement of region distinct biological processes, of which pathways implicated in cellular respiratory metabolism were overarching features. In the VWM frontal white matter, proteome changes were associated with decrease in glycolysis/gluconeogenesis and metabolism of various amino acids. By contrast, in the VWM pons white matter, we found a decrease in oxidative phosphorylation. Taken together, our data show that brain regions are affected in parallel in VWM, but to different degrees. We found region-specific involvement of different cell types and discovered that cellular respiratory metabolism is differently affected across white matter regions in VWM. These region-specific changes help explain regional vulnerability to pathology in VWM.

2023-06-28 | PXD040861 | Pride

Bulk RNAseq data of white matter tissue injury

Project description:We compared the transcriptomic responses in the mouse white matter tissue drive white matter lesions following LPC- and L-NIO-induced injury.

2025-12-08 | GSE312001 | GEO

Spatial transcriptomics of the aging mouse brain reveals origins of inflammation in the white matter

Project description:Spatial transcriptomics of the aging mouse brain reveals origins of inflammation in the white matter

| PRJNA979150 | ENA