Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell gene expression of a glioma cell line (GL261) cultured adherently or as neurospheres

ABSTRACT: Single-cell gene expression of a glioma cell line (GL261) cultured adherently or as neurospheres

PROVIDER: PRJNA1164322 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Single-cell gene expression of a glioma cell line (GL261) cultured adherently or as neurospheres

Project description:GL261 cells are a widely-used model system for glioma tumors and can be cultured adherently or as free-floating cell aggregates known as neurospheres. Neurospheres can be implanted into mice where they readily form tumors. This suggests that neurospheres may more closely resemble the in-vivo tumor phenotype. Our study has two primary objectives: 1) To determine differences in gene expression between adherent cells and the tumor-forming neurospheres and 2) To determine the extent of homogeneity in these cell populations. This project was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health (Grant #P20GM103408) along with a Murdock College Science Research Program Grant (SR-202119904) from the M.J. Murdock Charitable Trust.

2025-08-18 | GSE277850 | GEO

RNAseq analysis of GL261 GDC and mNS GSC transcriptome

Project description:We compare the gene expression profile between GL261 glioma differentiated cells (GDC) and mNS Neurospheres glioma stem like cells (GSC).

2017-02-03 | GSE94239 | GEO

Primarily cultured neurospheres

Project description:Primarily cultured neurospheres

| PRJNA599138 | ENA

RNA-seq of co-culture experiment between human endothelial cells (HUVECs) and mouse glioma (Gl261) with or without Notch Inhibitor Gamma Secretase Inhibitor (GSI)

Project description:Increased awareness for the role for angiocrine factors in stem cell biology, in general, has suggested a role for angiocrine factors in the maintenance of GSC stemness and also there are reciprocal bi-directional signals from endothelial cells (ECs) to glioma (Gl261) and vice versa. To extend, refine and elucidate perfusion-independent modes of GBM-EC communication (i.e., distinguishing contact dependent or contact independent routes), we carried-out the following experiments: ECs from umbilical veins tagged with RFP (HUVEC-RFP) were cultured with mouse glioma line Gl261 fluorescently labelled with GFP (Gl261-GFP) until reaching confluency by 72 h post culturing. Cells were than harvested, RNA extracted, and the combined co-culture transcriptomes were sequenced from a generated cDNA library without prior separation of the respective cell types (sample named as Co). The Co transcriptome was compared to that of an artificial mixtures of the two cell types (Gl261-GFP and HUVEC-RFP) in the exact cells ratio attained by the end of the co-cluttering aided by FACS-based determination of the GFP/RFP ratio (sample named as Sep). In this procedure advantage was taken on the different species from which GBM and ECs are derived and specialized bioinformatics tools allowing to unambiguously assign the transcripts to their respective GBM (mouse) or EC (human) origins, thereby circumventing confounding factors associated with cell purification. Considering that contact-dependent EC signaling is primarily regulated by canonical Notch pathway and that the Notch pathway was reported to play a role in self-renewal of GSCs, we also treated GBM-EC co-cultures with the Notch pathway inhibitor Gamma secretase inhibitor (GSI) (or with the DMSO vehicle alone) for 24 h before harvesting.

2022-01-01 | E-MTAB-9604 | biostudies-arrayexpress

Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs

Project description:GL261 cells are one of the most common mouse glioblastoma cells used for in vivo studies in a immunocompetent model. We analyze GL261 and stem cells derived from them (GL261-GSCs) using scRNA-Seq.

2024-03-28 | GSE244301 | GEO

Extra deep sequencing of GL261 and LLC1 murine cell lines for the purpose of fusion detection

Project description:RNA from GL261 (glioma) and LLC1 (Lewis Lung carcinoma) samples were sequenced at a depth of 142M and 188M paired reads, respectively.

2019-01-23 | GSE97975 | GEO

Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs

Project description:Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs

| PRJNA1022144 | ENA

Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs

Project description:Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs

| PRJNA1032148 | ENA

Effects of CBD on the glioma stem cell molecular signature

Project description:Primary glioma stem cells cultured as neurospheres in NBL media with growth factors were subjected to treatment with the non-toxic, non-psychoactive cannabis compound cannabidiol (CBD). Control and CBD- treated cultures were used to generate RNA used to hybridize on Affymetrix DNA arrays

2015-04-17 | GSE57978 | GEO

Mouse neurospheres

Project description:Seven day old platelet-derived growth factor-generated neurospheres cultured from the embryonic ganglia Keywords: other

2008-03-01 | GSE10614 | GEO