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The response of human coronary artery endothelial cells to PPBP treatment

ABSTRACT: The response of human coronary artery endothelial cells to PPBP treatment

PROVIDER: PRJNA1164712 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30841340_1.fastq.gz	Fastqsanger.gz
	SRR30841340_2.fastq.gz	Fastqsanger.gz
	SRR30841341_1.fastq.gz	Fastqsanger.gz
	SRR30841341_2.fastq.gz	Fastqsanger.gz
	SRR30841342_1.fastq.gz	Fastqsanger.gz

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The response of human coronary artery endothelial cells to PPBP treatment

Project description:Pro-platelet basic protein (PPBP), a platelet-derived member of the CXC chemokine family, activates neutrophils and other immune cells via CXCR1 and CXCR2 receptors. It is associated with various diseases, including chronic obstructive pulmonary disease, inflammatory bowel disease, and rheumatoid arthritis. In our study, PPBP was found to be significantly upregulated in both the dermal adipose tissue and plasma of patients with psoriasis. Administration of PPBP or imiquimod in Apoe-/- mice exacerbated atherosclerosis by inducing oxidative stress and mitochondrial dysfunction in coronary artery endothelial cells. Conversely, neutralizing PPBP or administering the mitochondria-targeted antioxidant Mito-TEMPO significantly reduced atherosclerotic plaque formation in these mice. To further explore the mechanisms by which PPBP contributes to atherosclerotic plaque development, we conducted transcriptomic analysis of human coronary artery endothelial cells (HCAEC) treated with PPBP

2025-06-07 | GSE277891 | GEO

Geometric control of the coronary artery endothelial cell transcriptome following standard growth condition

Project description:Analysis of the effects of cell shape on human coronary artery endothelial cell transcription. The hypothesis is that defined alterations in endothelial cell shape uniquely affect the endothelial transcriptome. Human coronary artery endothelial cells were plated onto spatially defined micropatterns (Cytoo) forcing them to conform to Disc, Crossbow, H, Y, or L shapes. As a control, human coronary artery endothelial cells were plated on non-restrictive areas of the Cytoo growth plate. RNA was collected after 24 hours growth on the restricted or non-restricted growth patterns and subjected to whole genome microarray analysis.

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Project description:Analysis of the effects of cell shape on human coronary artery endothelial cell transcription. The hypothesis is that defined alterations in endothelial cell shape uniquely affect the endothelial transcriptome. Human coronary artery endothelial cells were plated onto spatially defined micropatterns (Cytoo) forcing them to conform to Disc, Crossbow, H, Y, or L shapes. As a control, human coronary artery endothelial cells were plated on non-restrictive areas of the Cytoo growth plate. Cells were serum starved for 48 hours prior to mRNA collection.

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Project description:Currently, it is well established that human endothelial cells (ECs) are characterised by a significant heterogeneity between distinct blood vessels, e.g., arteries, veins, capillaries, and lymphatic vessels. Further, even ECs belonging to the same lineage but grown under different flow patterns (e.g., laminar and oscillatory or turbulent flow) ostensibly have distinct molecular profiles defining their physiological behaviour. Human coronary artery endothelial cells (HCAEC) and human internal thoracic artery endothelial cells (HITAEC) represent two cell lines inhabiting atheroprone and atheroresistant blood vessels (coronary artery and internal thoracic artery, respectively). Resistance of the internal mammary artery to atherosclerosis has been largely attributed to the protective phenotype of HITAEC which reportedly produce higher amounts of vasodilators including nitric oxide (NO) through the respective signaling pathways. However, this hypothesis has not been adequately addressed hitherto as proteomic profiling of HCAEC and HITAEC in a head-to-head comparison setting has not been performed.

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