Project description:Pleurotus species include edible mushrooms that can grow on a broad range of lignocellulosic substrates by secreting a coordinated system of hydrolytic and oxidative enzymes. This flexibility offers opportunities to valorize agro-industrial residues for production of food and biomaterials using sustainable processes. In this study, we analyzed the genome of Pleurotus pulmonarius LGAM 28684 and investigated its biocatalytic potential for saccharification of pretreated lignocellulose using LC-MS/MS proteomics and biochemical assays. The fungus was cultivated on two lignocellulosic substrates, beechwood and corn stover, as well as xylose as a control. The fungus was cultivated on beechwood, corn stover, and xylose. Beechwood induced the richest secretome with abundant oxidases. The corn stover secretome had fewer proteins but was focused on carbohydrate-acting enzymes, with abundant polysaccharide-degrading and accessory enzymes. Despite lower enzyme diversity, the corn stover secretome achieved higher lignocellulose saccharification, further improved by oxidoreductase inhibition. Supplementing an industrial cellulase cocktail with P. pulmonarius corn stover secretomes enhanced sugar release by 40%. These findings highlight the dynamic enzymatic response of P. pulmonarius to lignocellulosic substrates and its potential in biomass valorization and the design of enzymatic cocktails.
2025-11-03 | PXD065010 | Pride
Project description:Activated electric field promotes hydrogen production from anaerobic dark fermentation
Project description:The fermentation of Zymomonas mobilis 2032 was conducted in 6% and 9% glucan-loading AFEX-pretreated corn stover hydrolysates, as well as in 7% glucan-loading AFEX-pretreated switchgrass hydrolysate, and RNA samples were collected at both glucose and xylose growth stages. Here we present a comprehensive RNA-Seq data set at both growth stages.
2022-04-24 | GSE201229 | GEO
Project description:Optimization of hydrogen-methane co-production from corn stover via enzymatic hydrolysis:
Project description:L-Lactic acid is a key monomer for polylactic acid (PLA), and its market demand continues to grow. Producing lactic acid from lignocellulosic feedstocks instead of grain can alleviate competition for food resources; however, inhibitors generated during pretreatment result in low fermentation titers and complex downstream processes, hindering industrial implementation. In this study, Pediococcus acidilactici NHRI04 with robust inhibitor tolerance was obtained through staged adaptive domestication, enabling direct fermentation of undetoxified corn stover hydrolysate. Process optimization achieved a final L-lactic acid concentration of 226.09 g/L, with a productivity of 3.12 g/L/h and a sugar-to-lactic acid yield of 99.72%, corresponding to an overall yield of 0.32 g/g corn stover and 98.80% optical purity. This titer represents the highest lactic acid concentration reported to date from lignocellulosic biomass. A pasteurization-based fermentation strategy (65 °C, 30 min) was established to replace conventional autoclaving, effectively preserving medium nutritional value. Economic analysis revealed that combining high-concentration fermentation, pasteurization, and detoxification elimination reduced production costs by 2,232 CNY per ton of lactic acid. Substituting corn stover for corn grain saves 1.8 tons of corn and reduces carbon emissions by 1.83 tons CO₂e per ton of lactic acid. Integrated whole-genome, transcriptomic, and proteomic analyses elucidated the molecular mechanisms underlying inhibitor tolerance. This study provides a feasible approach for low-cost, grain-saving, and low-carbon production of lignocellulosic lactic acid.
Project description:In the present work, Abortiporus biennis, a white-rot fungus, was studied in regard to its lignocellulolytic enzymatic potential. Secretomics analyses, combined with biochemical methods, were employed to study the enzymatic machinery of the strain, after growth in corn stover cultures and xylose-based defined media. The results revealed the presence of all the necessary enzymatic activities for complete breakdown of the lignocellulosic substrate, while the prominent role of oxidative enzymes in the lignocellulolytic strategy of the strain became evident. Two novel laccases, AbiLac1 and AbiLac2, were isolated from the culture supernatant of this fungus.
Project description:Furans (furfural and 5-hydroxymethylfurfural (HMF)), phenolic aldehydes (4-hydroxybenzaldehyde, syringaldehyde, and vanillin), and weak acids (acetic acid and formic acid) are the main degradation products of lignocellulose pretreatment process and seriously inhibit the cellullas enzyme activity and the fermentation process.
2022-07-21 | GSE208427 | GEO
Project description:Hydrogen production through lactate-driven dark fermentation
