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Bos taurus breed:Holstein

ABSTRACT: Host transcriptome response to Mycoplasma bovis and bovine viral diarrhea virus in bovine tissues

PROVIDER: PRJNA1166195 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31176300_1.fastq.gz	Fastqsanger.gz
	SRR31176300_2.fastq.gz	Fastqsanger.gz
	SRR31176301_1.fastq.gz	Fastqsanger.gz
	SRR31176301_2.fastq.gz	Fastqsanger.gz
	SRR31176302_1.fastq.gz	Fastqsanger.gz

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Project description:The immune response associated with mastitis caused by Mycoplasma bovis is a very complicated biological process in several type of cells, including immune cells, mammary epithelial cells and, endothelial cells. Thus, revealing of the microRNAs in the Mycoplasma bovis infected mammary gland tissues is particularly important for the immune response mechanism to Mycoplasma bovis. Firstly, mammary gland tissue samples were collected from Holstein cows and screened for Mycoplasma bovis. Then, total RNA was isolated from mycoplasma bovis infected tissues and RNA sequencing was performed. After bioinformatics analysis, GO and KEGG analysis of target genes of identified microRNAs were conducted. Our results revaled that 24 of the known microRNAs were expressed differently and 13 of the novel microRNAs were expressed differently in Mycoplasma bovis positive tissues. The target genes of these microRNAs were found to be associated with especially inflammation pathways. In conclusion, this study demonstrated that identified miRNAs may be involved in the signaling pathways during mastitis case caused by Mycoplasma bovis.

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