Engineering Fibroblast with Reprogramming and Spheronization for Bone Defect Repair
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ABSTRACT:
PROVIDER: PRJNA1168097 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
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| SRR30863101_1.fastq.gz | Fastqsanger.gz | |||
| SRR30863101_2.fastq.gz | Fastqsanger.gz | |||
| SRR30863102_1.fastq.gz | Fastqsanger.gz | |||
| SRR30863102_2.fastq.gz | Fastqsanger.gz | |||
| SRR30863103_1.fastq.gz | Fastqsanger.gz |
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Project description:Engineering Fibroblast with Reprogramming and Spheronization for Bone Defect Repair
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Project description:Repairing bone defects is a complex cascade reaction process, as immune system regulation, vascular growth, and osteogenic differentiation are essential. Thus, developing a tissue-engineered biomaterial that caters to the complex healing process of bone regeneration remains a major clinical challenge. In the study, Ca2+-TA-rGO (CTAG)/GelMA hydrogels were synthesized by binding Ca2+ using metal chelation to graphene oxide (GO) nanosheets reduced by tannic acid (TA-rGO) and doping them into gelatin methacrylate (GelMA) hydrogels. TA and rGO exhibited biocompatibility and immunomodulatory properties in this composite, while Ca2+ promoted bone formation and angiogenesis. This novel nanocomposite hydrogel demonstrated good mechanical properties, degradability, and conductivity, and it could achieve slow Ca2+ release during bone regeneration. Both in vitro and in vivo experiments revealed that CTAG/GelMA hydrogel modulated macrophage reprogramming and induced a shift from macrophages to healing-promoting M2 macrophages during the inflammatory phase, promoted vascular neovascularization, and facilitated osteoblast differentiation during bone formation. Moreover, CTAG/GelMA hydrogel could downregulate the NF-κB signaling pathway, offering new insights into regulating macrophage reprogramming-osteogenic crosstalk. Conclusively, this novel multifunctional nanocomposite hydrogel provides a multistage treatment for bone and orchestrates macrophage reprogramming-osteogenic crosstalk to boost bone repair.
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