Project description:RNA-seq on 120hr L3 larval wing imaginal discs. 3 replicates of dis3L2 null mutant, and 3 replicates of control wing discs. rRNA depleted, Illumina TruSeq libraries. Paired-end sequencing on a HiSeq 3000.
Project description:RNA-sequencing polyA library preparation in wing discs (WD) and pupal wings (PW) from inbred (D907) and outbred (Mix) fly populations from lines selected for the ectopic veins trait under genetic assimilation (assimilated) or direct artificial selection under normal conditions (non-assimilated) and unselected (control) flies.
Project description:We performed RNA sequencing of wing discs at the wandering L3 larval stage from 32 inbred lines of Drosophila genetic reference panel (DGRP) that consists of 16 big and 16 small wing lines. We aimed to understand system-wide gene regulatory mechanisms that attain the observed natural variation in wing size including the sexual size dimorphism.
Project description:During the last larval stage (L3) of Drosophila melanosgaster development, wing imaginal discs undergo tremendous growth and initiate differentiation programs. This transcriptomic time course aims at uncovering the the genes that are temporally regulated during the L3 in wing imaginal discs to regulate the dynamics of growth and differentiation.
Project description:Screening for binding partners of the splicing factor SmD3 and changes in interaction upon depletion of the protein Ecdysoneless (Ecd) in the nubbin domain of third-instar larval wing imaginal discs.