Apis cerana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1169767 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR30983800_1.fastq.gz | Fastqsanger.gz | |||
| SRR30983800_2.fastq.gz | Fastqsanger.gz | |||
| SRR30983801_1.fastq.gz | Fastqsanger.gz | |||
| SRR30983801_2.fastq.gz | Fastqsanger.gz |
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Project description:High-throughput RNA sequencing was carried out for understanding of caste and organs of Asian honeybees.
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Project description:High-throughput RNA sequencing was carried out for understanding of caste and organs of Asian honeybees.
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Project description:Apis cerana honeybees' hypopharyngeal glands DGE project
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Project description:Natural history museum specimens of historical honeybees have been successfully used to explore the genomic past of the honeybee, indicating fast and rapid changes between historical and modern specimens, possibly as a response to current challenges. In our study we explore a potential untapped archive from natural history collections - specimens of beeswax. We examine an Apis mellifera mellifera queen cell specimen from the 19th century. The intact and closed cell was analysed by X-ray Computed Tomography (CT) to reveal a perfectly preserved queen bee inside her cell. Subsequently, a micro-destructive approach was used to evaluate the possibility of protein extraction from the cell. Our results show that studies on specimens such as these provide valuable information about the past rearing of queens, their diet and development, which is relevant for understanding current honeybee behaviour. In addition we evaluate the feasibility of using historical beeswax as a biomolecular archive for ancient proteins to study honeybees.
2024-09-05 | PXD034106 | Pride
Project description:BackgroundThe Eastern hive honey bee, Apis cerana cerana is a native and widely bred honey bee species in China. Molecular biology research about this honey bee species is scarce, and genomic information for A. c. cerana is not currently available. Transcriptome and expression profiling data for this species are therefore important resources needed to better understand the biological mechanisms of A. c. cerana. In this study, we obtained the transcriptome information of A. c. cerana by RNA-sequencing and compared gene expression differences between queens and workers of A. c. cerana by digital gene expression (DGE) analysis.ResultsUsing high-throughput Illumina RNA sequencing we obtained 51,581,510 clean reads corresponding to 4.64 Gb total nucleotides from a single run. These reads were assembled into 46,999 unigenes with a mean length of 676 bp. Based on a sequence similarity search against the five public databases (NR, Swissport, GO, COG, KEGG) with a cut-off E-value of 10(-5) using BLASTX, a total of 24,630 unigenes were annotated with gene descriptions, gene ontology terms, or metabolic pathways. Using these transcriptome data as references we analyzed the gene expression differences between the queens and workers of A. c. cerana using a tag-based digital gene expression method. We obtained 5.96 and 5.66 million clean tags from the queen and worker samples, respectively. A total of 414 genes were differentially expressed between them, with 189 up-regulated and 225 down-regulated in queens.ConclusionsOur transcriptome data provide a comprehensive sequence resource for future A. c. cerana study, establishing an important public information platform for functional genomic studies in A. c. cerana. Furthermore, the DGE data provide comprehensive gene expression information for the queens and workers, which will facilitate our understanding of the molecular mechanisms of the different physiological aspects of the two castes.
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Project description:To obtain the presence of environmental contaminants in honeybee and compare the toxicity of the detected pesticides to Apis mellifera ligustica Spin and Apis cerana cerana Fabricius. In this work, 214 honeybee samples were collected to simultaneous monitoring 66 pesticides between 2016 and 2017 in China. A modified QuEChERS extraction method coupled with multi-residue analytical methods by Ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and Gas chromatography-mass spectrum (GC-MS). Among, four pyrethroid pesticides were selected to test and compare the acute oral toxicities of two honeybees. And the survival risk of beta-cypermethrin was analyzed to them. Using this method, 21 compounds were detected, including 3 neonicotinoids, 5 pyrethroids, 5 organophosphorus and 8 others. Importantly, detected frequencies of pyrethroid pesticides were accounted for 53.3%. Among, acute toxicity values (LD50) of four pyrethroid pesticides to the A.m. ligustica were higher than of that the A.c. cerana. When they were exposed to the same concentration of beta-cypermethrin (0.2906 mg/L), the survival rate of the A.m. ligustica (40.0%) was higher than the A.c. cerana (18.9%). Our work is valuable to analyze multiple pesticide residues of honeybees and evaluate the survival risk of two honeybee species, which also provides a basis for the risk assessment.
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Project description:As detoxification enzymes, proteins in the glutathione S-transferase (GST) superfamily are reported to participate in oxidative stress resistance. Nevertheless, microsomal GSTs (MGSTs), a unique subclass of the GST superfamily associated with membranes, are rarely studied in insects. Here, we isolated an MGST gene in Apis cerana cerana (AccMGST1) and verified its role in oxidative stress response. We found higher expression of AccMGST1 in protective or defensive tissue, that is, the epidermis, which indicated its role in stress resistance. Real-time quantitative PCR (qRT-PCR) analysis indicated that AccMGST1 was upregulated by oxidative stresses at the transcriptional level. In contrast, AccMGST1 expression was inhibited when the antioxidant vitamin C (VC) was fed to experimental bees. Through western blotting, we found that the protein level of AccMGST1 under oxidative stress corresponded to the transcript level. Disc diffusion and mixed-function oxidation (MFO) assays suggested that AccMGST1 can protect not only cells but also DNA against oxidative damage. Furthermore, we discovered that the expression patterns of known antioxidant genes were changed in A. cerana cerana after AccMGST1 was silenced by RNA interference (RNAi). Thus, we concluded that the gene AccMGST1 exerts a significant role in the antioxidant mechanism.
| S-EPMC6629756 | biostudies-literature
Project description:Sacbrood virus(SBV) is one of the most destructive viruses in the Asian honeybee Apis cerana but is much less destructive in Apis mellifera In previous studies, SBV isolates infecting A. cerana(AcSBV) and SBV isolates infecting A. mellifera(AmSBV) were identified as different serotypes, suggesting a species barrier in SBV infection. In order to investigate this species isolation, we examined the presence of SBV infection in 318A. mellifera colonies and 64A. cerana colonies, and we identified the genotypes of SBV isolates. We also performed artificial infection experiments under both laboratory and field conditions. The results showed that 38A. mellifera colonies and 37A. cerana colonies were positive for SBV infection. Phylogenetic analysis based on RNA-dependent RNA polymerase (RdRp) gene sequences indicated that A. cerana isolates and most A. mellifera isolates formed two distinct clades but two strains isolated fromA. mellifera were clustered with theA. cerana isolates. In the artificial-infection experiments, AcSBV negative-strand RNA could be detected in both adult bees and larvae ofA. mellifera, although there were no obvious signs of the disease, demonstrating the replication of AcSBV inA. mellifera Our results suggest that AcSBV is able to infectA. melliferacolonies with low prevalence (0.63% in this study) and pathogenicity. This work will help explain the different susceptibilities ofA. cerana and A. melliferato sacbrood disease and is potentially useful for guiding beekeeping practices.
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Project description:Apis cerana cerana larvae Genome sequencing and assembly
| PRJNA760941 | ENA