Project description:Glucocorticoids act in a highly coordinated manner on various tissues, causing discrete patterns of biological effects. In this study, we aimed to inspect this very sophisticated transcriptional machine by comparing gene expression changes in specific tissues after the administration of a prototypical GR (dexamethasone). We reevaluate the tissue-specific acute effects of dexamethasone on gene expression. We provide a resource summarizing the effects on gene expression of dexamethasone in multiple tissues in mice, as a model organism.
Project description:We used microarrays to identify transcripts regulated by dexamethasone in omental (Om) and abdominal subcutaneous (Abdsc) adipose tissues of severely obese females obtained during elective surgeries. Minced tissue was treated with Dexamethasone in organ culture for 7 days as previously described (Lee et al, Am J Physiol Endocrinol Metab. 2011 Mar;300(3):E571-80).