Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide DNA replication profiling and full-length total RNA sequencing from the same single cell [02_Mouse8cell_embryos]

ABSTRACT: Genome-wide DNA replication profiling and full-length total RNA sequencing from the same single cell [02_Mouse8cell_embryos]

PROVIDER: PRJNA1169807 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Genome-wide DNA replication profiling and full-length total RNA sequencing from the same single cell [02_Mouse8cell_embryos]

Project description:Single-cell sequencing has advanced our understanding of cell-type diversity and heterogeneity. However, existing single-cell multi-omics methods lack DNA data resolution and full-length total RNA detection. Here, we introduce single-cell (sc)Repli-RamDA-seq (scRR-seq), a novel multi-omics method that enables high-resolution DNA replication profiling and full-length total RNA sequencing from the same single cell, made possible by the clean separation of DNA and RNA. scRR-seq generates DNA replication and RNA sequencing data comparable to individually obtained scRepli-seq and scRamDA-seq data, respectively. scRR-seq also allows one to tell the cell-cycle stage of a given S-phase cell and detects copy-number variation (CNV) in non-S-phase cells. scRR-seq outperforms other scRNA-seq methods in the number of transcripts identified and full-length total RNA detection. Furthermore, scRR-seq allowed haplotype-specific analysis and the identification of novel S-phase markers. Taken together, scRR-seq is a robust single-cell multi-omics method with promising potential for comprehensive genome and transcriptome analysis.

2025-08-27 | GSE278944 | GEO

Genome-wide DNA replication profiling and full-length total RNA sequencing from the same single cell [01_RPE1_midS]

2025-08-27 | GSE278941 | GEO

Genome-wide DNA replication profiling and full-length total RNA sequencing from the same single cell [03_CBMS1_mESC]

2025-08-27 | GSE278949 | GEO

Genome-wide DNA replication profiling and full-length total RNA sequencing from the same single cell [HAP1 mid-S]

2025-08-27 | GSE278952 | GEO

Genome-wide DNA replication profiling and full-length total RNA sequencing from the same single cell [IMR-90 G1]

2025-08-27 | GSE278958 | GEO

Genome-wide DNA replication profiling and full-length total RNA sequencing from the same single cell [hTERT-RPE1 Whole-S]

2025-08-27 | GSE278951 | GEO

Genome-wide DNA replication profiling and full-length total RNA sequencing from the same single cell