Project description:Single-cell transcriptome analysis reveals distinct cell populations in dorsal root ganglia and their potential roles in diabetic peripheral neuropathy
Project description:The peripheral nervous system (PNS) orchestrates organ function during homeostasis and stress. Most cancers including pancreatic ductal adenocarcinoma (PDAC) are infiltrated by PNS neurons participating in their complex tumor microenvironment. Here, we analyze the effect of neuronal interactions with fibroblasts and cancer cells with dorsal root and celiac ganglia. To this end, both cell types were co-cultured with both peripheral ganglia and subjected to bulk RNA-Sequencing.
Project description:In the peripheral nervous system, the prevertebral ganglion (PVG) serves as an important relay station, transmitting centrifugal signals to visceral organs; the PVG is also known to innervate enteroanal afferent neurons (IFANs) and spinal sensory neurons innervating the intestinal tract. It has been suggested that there are neural circuits within the PVG consisting of sensory and sympathetic neurons. Here, we used a single nuclei RNA sequencing method to characterize the gene expression profiles of individual cells constituting the ventral ganglion in normal rats. These data provide valuable material for examining the neural circuits within the PVG.
Project description:The goal of this study was to analyze global gene expression in specific populations of nociceptor sensory neurons, the neurons that detect damaging/noxious stimuli. The dorsal root ganglia (DRG), trigeminal ganglia, and nodose ganglia are anatomically distinct peripheral sensory ganglia that contain nociceptors which innervate skin, gut, lungs, and other distinct organ tissues. We used flow cytometry to purify nociceptors from these ganglia and profiled their global gene expression signatures to compare gene expression between these different anatomically distinct nociceptors.
Project description:The goal of this study was to analyze global gene expression in specific populations of nociceptor sensory neurons, the neurons that detect damaging/noxious stimuli. The dorsal root ganglia (DRG), trigeminal ganglia, and nodose ganglia are anatomically distinct peripheral sensory ganglia that contain nociceptors which innervate skin, gut, lungs, and other distinct organ tissues. We used flow cytometry to purify nociceptors from these ganglia and profiled their global gene expression signatures to compare gene expression between these different anatomically distinct nociceptors. Nav1.8-Cre were bred with Rosa26-TdTomato to generate Nav1.8-Cre/R26-TdTomato reporter progeny, where all peripheral nociceptor neurons are genetically marked with red fluroescence due to specific expression of the TTX- resistant sodium channel Nav1.8. Lumbar region dorsal root ganglia (DRG), trigeminal ganglia, and nodose ganglia were dissected from mice (3 mice were pooled/sample). Highly red fluorescent neurons were Facs purified, RNA extracted, and processed for microarray analysis.
Project description:Single cell RNAseq was performed on naïve adult mouse lumbar dorsal root ganglia (DRG) cells. Neuronal and non-neuronal cell populations were identified.