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Germline transmission of cryopreserved mouse spermatogonial stem cells maintained on the International Space Station

ABSTRACT: Germline transmission of cryopreserved mouse spermatogonial stem cells maintained on the International Space Station

PROVIDER: PRJNA1171796 | ENA |

REPOSITORIES: ENA

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Germline transmission of cryopreserved mouse spermatogonial stem cells maintained on the International Space Station

Project description:Spermatogonial stem cells (SSCs) undergo self-renewal division to maintain spermatogenesis. In this study, we used SSCs for long-term cryopreservation in space flight. We derived germline stem cell (GS) cultures from DBA/2 mouse testes by supplementing GDNF and FGF2 (Kanatsu-Shinohara et al., 2003, Biol. Reprod. 69, 216-616). GS cells were cryopreserved in the ISS for 6 months. Frozen GS cells were thawed upon return to Earth and their gene expression profiles were analysed.

2025-08-12 | GSE279277 | GEO

Expression data from cultured mouse spermatogonia cryopreserved on the International Space Station (ISS) or on Earth

2025-08-15 | GSE280403 | GEO

Expression data from adult liver of offspring derived from cultured spermatogonia (GS cells) cryopreserved for a 6 months on the International Space Station (ISS) or on Earth.

Project description:Spermatogonial stem cells (SSCs) undergo self-renewal division to maintain spermatogenesis. In this study, we used SSCs for long-term cryopreservation in space flight. We derived germline stem cell (GS) cultures from DBA/2 mouse testes (Kanatsu-Shinohara et al., 2003, Biol. Reprod. 69, 216-616). GS cells were cryopreserved in the ISS for 6 months. Frozen GS cells were thawed upon return to Earth and tranplanted into the seminifelous tubules of infertile W mice. Recipients were housed with wild-type C57BL/6 females, and livers of F1 offspring were analyzed for gene expression.

2025-08-15 | GSE280404 | GEO

Microgravity on the International Space Station promotes survival and proliferation of human stem cell cardiomyocytes

Project description:Efficient generation of functional cardiomyocytes from human induced pluripotent stem cells (hiPSC-CMs) is critical for their use in regenerative medicine and other applications. In this study, we evaluated the effect of space microgravity (µg) on the differentiation of hiPSC-derived cardiac progenitors compared with parallel 1g condition on the International Space Station. Cryopreserved 3D cardiac progenitors derived from hiPSCs were cultured for 3 weeks. Compared with 1g culture, the µg culture had larger sphere sizes, increased expression of proliferation markers, higher counts of nuclei, and higher cell viability. Highly enriched cardiomyocytes generated in µg had appropriate gene expression and cardiac structure as well as improved function including contractility and Ca2+ handling. RNA-seq analysis of 3-day cultures revealed that short-term exposure of cardiac progenitor spheres to space microgravity upregulated genes involved in cell proliferation, cardiac differentiation, and contraction. These results indicate that space microgravity increased survival and proliferation of hiPSC-CMs and improved their structures and functions.

2022-09-08 | GSE188793 | GEO

Expression data from cultured mouse spermatogonia cryopreserved on the International Space Station (ISS) or on Earth

Project description:Expression data from cultured mouse spermatogonia cryopreserved on the International Space Station (ISS) or on Earth

| PRJNA1178319 | ENA

Swabs from the International Space Station

Project description:Swabs from the International Space Station Raw sequence reads

| PRJNA376404 | ENA

SCD – Stem Cell Differentiation on board the International Space Station [RNA-seq]

Project description:The bone loss observed in astronauts and animal models after spaceflight is attributable to alterations in the bone tissue formation that depends from the continuous remodeling through the activities of bone-resorbing osteoclasts of hematopoietic lineage and bone-forming osteoblasts of mesenchymal origin. This disease is frequent in aged people, but develops much more rapidly in space. Our experiment, selected by ESA (European Space Agency), aimed to determine how human bone marrow mesenchymal stem cells (hBMSCs) react and differentiate in real microgravity, on board the International Space Station, in approx. 2 weeks time.

2019-01-31 | GSE100931 | GEO

SCD – Stem Cell Differentiation on board the International Space Station [microarray on total RNA]

2019-01-31 | GSE100930 | GEO

Effects of a Closed Space Environment on Gene Expression in Hair Follicles of Astronauts in the International Space Station

2016-03-31 | GSE74708 | GEO

Effects of a Closed Space Environment on Gene Expression in Hair Follicles of Astronauts in the International Space Station

Project description:In recent times, long-term stay has become a common occurrence in the International Space Station (ISS). However, adaptation to the space environment can sometimes pose physiological problems to the astronauts after their return. Therefore, it is important to develop healthcare technologies for astronauts. In this study, hair, an easy-to-obtain sample, was identified as the candidate. In order to investigate the genetic changes in human hair during space flight, the hair follicles of 10 astronauts were analyzed by DNA microarray and real time q-PCR analyses. Space environment induced gene expression of hair follicles of astronaut was measured 6 differnent times included 2 in flight on orbit. Ten independent experiments were performed on differing astronauts. and the sampling day was differed for each astronaut because of their schedules.

2016-03-31 | E-GEOD-74708 | biostudies-arrayexpress

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