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In vivo RNA sequencing reveals a crucial role of Fus3-Kss1 MAPKpathway in Candida glabrata pathogenicity

ABSTRACT: In vivo RNA sequencing reveals a crucial role of Fus3-Kss1 MAPKpathway in Candida glabrata pathogenicity

PROVIDER: PRJNA1171806 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30951835_1.fastq.gz	Fastqsanger.gz
	SRR30951835_2.fastq.gz	Fastqsanger.gz
	SRR30951836_1.fastq.gz	Fastqsanger.gz
	SRR30951836_2.fastq.gz	Fastqsanger.gz
	SRR30951837_1.fastq.gz	Fastqsanger.gz

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In vivo RNA sequencing reveals a crucial role of Fus3-Kss1 MAPKpathway in Candida glabrata pathogenicity

Project description:Candida glabrata is an important and increasingly common pathogen of humans, particularly in immunocompromised hosts. Despite this, little is known about how this fungus causes disease. Here, we applied RNA sequencing and an in vivo invasive infection model to identify the attributes that allow this organism to infect hosts. Fungal transcriptomes show a dramatic increase in the expression of Fus3 and Kss1, two mitogen-activated protein kinases (MAPKs), during invasive infection. We further demonstrate that they are both highly induced under a combination of serum and high CO2 condition. Deletion of both FUS3 and KSS1, but neither gene alone, results in a reduced fungal burden in organs, as well as in gastrointestinal tract in the DSS (Dextran Sulfate Sodium)-induced colitis model. Similarly, the defect in persistence in macrophages and attenuated adhesion to epithelial cells are observed when FUS3 and KSS1 are both disrupted. The fus3 kss1 double mutant also displays defects in the induction of virulence attributes such as genes required for iron acquisition and adhesion, and in the anti-fungal drug tolerance. The putative downstream transcription factors Ste12(1), Ste12(2), Tec1 and Tec2 are found to be involved in the regulation of these virulence attributes. Collectively, our study indicates that an evolutionary conserved MAPK pathway which regulates mating and filamentous growth in Saccharomyces cerevisiae, is critical for C. glabrata pathogenicity.

2024-10-16 | GSE279281 | GEO

Translation Regulation Promotes Stress Adaptation in Candida glabrata

Project description:Candida glabrata is an important human fungal pathogen leading cause of non-albicans Candida infections. C. glabrata exhibits resistance to key antifungal drugs, rapidly replicates and divides in host macrophages and withstands highly stressful host conditions. This study explores the molecular mechanisms underlying stress adaptations in C. glabrata that contribute to its pathogenicity. Our findings revealed that C. glabrata survives oxidative stress and amino acid starvation more effectively than S. cerevisiae, C. albicans, and C. auris. We noted that Gcn2 kinase and Gcn4 play critical roles in this adaptation as Gcn2 phosphorylates eIF2α and downregulates the global protein translation, activating GCN4. RNA sequencing of WT and gcn4 mutant revealed that GCN4 activation during stress orchestrates the expression of stress-responsive genes vital for survival during amino acid starvation and oxidative stress. Ultimately assisting in the stress adaptative transcriptome. Thus, this study highlights the critical role of the Gcn2–Gcn4 pathway in stress adaptation in C. glabrata.

2025-07-14 | GSE283533 | GEO

Candida glabrata haploid and diploid

Project description:The different expression of Candida glabrata haploid and diploid.

2021-04-30 | GSE173617 | GEO

Homo sapiens whole blood infected with Candida spp. (here: Candida glabrata)

Project description:Homo sapiens fresh whole blood was infected with Candida glabrata. RNA-pool of both species extracted at 0min (control), 15, 30, 60, 120, 240 min. Samples are rRNA depleted. Measurement of Candida glabrata gene expression.

2018-12-26 | GSE114175 | GEO

Limosilactobacillus fermentum Limits Candida glabrata Growth by Ergosterol Depletion

Project description:Candida glabrata is a human-associated opportunistic fungal pathogen. It shares its niche with Lactobacillus spp. in the gastrointestinal and vaginal tract. In fact, Lactobacillus species are thought to competitively prevent Candida overgrowth. We investigated the molecular aspects of this antifungal effect by analyzing the interaction of C. glabrata strains with Limosilactobacillus fermentum. From a collection of clinical C. glabrata isolates, we identified strains with different sensitivities to L. fermentum in coculture. We analyzed the variation of their expression pattern to isolate the specific response to L. fermentum. C. glabrata-L. fermentum coculture induced genes associated with ergosterol biosynthesis, weak acid stress, and drug/chemical stress. L. fermentum coculture depleted C. glabrata ergosterol. The reduction of ergosterol was dependent on the Lactobacillus species, even in coculture with different Candida species. We found a similar ergosterol-depleting effect with other lactobacillus strains (Lactobacillus crispatus and Lactobacillus rhamosus) on Candida albicans, Candida tropicalis, and Candida krusei. The addition of ergosterol improved C. glabrata growth in the coculture. Blocking ergosterol synthesis with fluconazole increased the susceptibility against L. fermentum, which was again mitigated by the addition of ergosterol. In accordance, a C. glabrata Derg11 mutant, defective in ergosterol biosynthesis, was highly sensitive to L. fermentum. In conclusion, our analysis indicates an unexpected direct function of ergosterol for C. glabrata proliferation in coculture with L. fermentum.

2023-02-08 | GSE202656 | GEO

Absolute expression level of Candida glabrata genes

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Project description:Comparison of young and old Candida glabrata cells reveals regulation of genes involved in cell wall remodeling, sterol biogenesis, stress pathways

2017-04-24 | GSE85985 | GEO

Profiling of Candida glabrata during Phagocytosis by Neutrophils

Project description:Our purpose is to clarify the global transcriptional profile in Candida glabrata phagocytized in human neutrophil.

2012-03-01 | GSE14868 | GEO

Antifungal drug responses in two Candida glabrata strains

Project description:Transcriptomic responses of Candida glabrata strains, BG2 and CBS138, to two azole antifungal drugs, Fluconazole and Voriconazole.

2025-07-29 | GSE273379 | GEO

Absolute expression level of Candida glabrata genes

Project description:Total RNA versus genomic DNA hybridization on custom arrays designed for all Candida glabrata genes Total RNA was collected in mid-log phase from Candida glabrata cells grown in rich medium (abbreviated CM, in house recipe). RNA was then converted to cDNA, Cy3-labeled and hybridized competitively against Cy5 labeled genomic DNA from Candida glabrata

2010-07-06 | E-GEOD-22194 | biostudies-arrayexpress

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