Project description:Karrikins promote seed germination in Arabidopsis thaliana. Completion of germination (protrusion of the radicle) is not observed until ~72 h in dormant wildtype seed under these conditions. We used microarrays to examine karrikin-induced transcriptional changes after 24 h of imbibition. Transcriptional changes may indicate events leading to karrikin-induced germination or karrikin-specific markers.
Project description:Karrikins promote seed germination in Arabidopsis thaliana. Completion of germination (protrusion of the radicle) is not observed until ~72 h in dormant wildtype seed under these conditions. We used microarrays to examine karrikin-induced transcriptional changes after 24 h of imbibition. Transcriptional changes may indicate events leading to karrikin-induced germination or karrikin-specific markers. Primary dormant seed of wildtype (which can germinate and is promoted by KAR1) and ga1-3 (which cannot germinate, and is not promoted by KAR1) were imbibed on water-agar or 1 uM KAR1 in the light at 20 C and assayed for gene expression after 24 h. Three or four biological replicates per genotype/treatment.
Project description:au07-11_haiku - compare haiku mutants with wildtype - What are other genes involved in haiku pathway? - IKU1, IKU2 belong to same pathway which involved in seed development. Using array analysis try to find other components of this pathway. Keywords: gene knock out
Project description:This study aimed to characterize the effect of GIGANTEA loss of function on gene expression in Arabidopsis thaliana seedlings genome-wide. To this end, transcriptomic analyses using high-throughput sequencing were carried out in Col-0 wildtype and gi-2 loss-of-function mutants.
