Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Transcriptome of DM-a-KG treated cell line KGN

PROVIDER: PRJNA1173322 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR31008254_1.fastq.gz	Fastqsanger.gz
	SRR31008254_2.fastq.gz	Fastqsanger.gz
	SRR31008255_1.fastq.gz	Fastqsanger.gz
	SRR31008255_2.fastq.gz	Fastqsanger.gz
	SRR31008256_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Project description:H3K14ac and H3K56ac Cut&TAG analysis of DM-a-KG treated KGN cells

| PRJNA1173369 | ENA

Project description:H3K4me1, H3K4me3 and H3K9me2 Cut Tag analysis of DM-a-KG treated KGN cells and overexpression SLC1A5 in KGN cells

| PRJNA1244852 | ENA

Project description:Transcriptome of overexpression HDAC5 in cell line KGN

| PRJNA1173315 | ENA

M249 melanoma cells: Vemurafenib and Selumetinib resistant through BRAF double minutes vs. parental

Project description:CGH profiling of M249 melanoma cell line treated with step-wise increasing Vemurafenib and Selumetinib to develop resistance (VSR). The resistance mechanims was through BRAF amplification in double minute (DM) format. The control cell line is untreated M249.

2021-12-20 | GSE153526 | GEO

Project description:Transcriptome of overexpression SLC1A5 in KGN granulosa cell line

| PRJNA1172872 | ENA

testosterone application induced H3K27 deacetylation

Project description:Decreased H3K27 deacetylation after KGN cell was treated with 10 um testosterone for 24 h.

2024-08-19 | GSE272605 | GEO

Differentially expressed mRNAs after silencing lncRNA DDGC

Project description:We have completed the mRNA expression profiling of human granulosa-like tumor cell line KGN to reveal the differentially expressed mRNAs upon lncRNA DDGC knockdown.

2021-02-20 | GSE158555 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of 77-39 in ovarian cancer Transcriptomes

Project description:Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compareExamining the role of sirt3 inhibitor 77-39 in ovarian granulosa cancer cells,kgn cell line,cov434 cell line by RNA-seq

2025-05-01 | GSE293585 | GEO

Gene knockdown and overexpression of 402C>G FOXL2 in COV434 and KGN cells

Project description:Despite their distinct biology, granulosa cell tumours (GCTs) are treated the same as other ovarian tumours. Intriguingly, a recurring somatic mutation in the transcription factor Forkhead Box L2 (FOXL2) 402C>G has been found in nearly all GCTs examined. This investigation aims to identify the pathogenicity of mutant FOXL2 by studying its altered transcriptional targets. The expression of mutant FOXL2 was reduced in the GCT cell line KGN, and wildtype and mutant FOXL2 were overexpressed in the GCT cell line COV434. Comparisons were made between the transcriptomes of control cells and cells altered by FOXL2 knockdown and overexpression, to detect potential transcriptional targets of mutant FOXL2. Comparisons were made between the transcriptomes of control cells and cells altered by FOXL2 knockdown and overexpression, to detect potential transcriptional targets of mutant FOXL2. Mutant FOXL2 was silenced in KGN cells with the use of siRNA and a non-targetting siRNA control. Wildtype and mutant FOXL2 were overexpressed in COV434 cells with the use of overexpression plasmids and an empty plasmid control. Each experiment was performed in triplicate. Total RNA was harvested at 24h post transfection and hybridised to Affymetrix U133 plus 2.0 arrays.

2012-11-05 | E-GEOD-39890 | biostudies-arrayexpress

Mitochondrial dynamics in ovarian granulosa cells during follicular growth and in human KGN cells

Project description:In the ovary, proliferation and differentiation of granulosa cells (GCs) drive the growth of follicles. This is, in part, dependent on gonadotropins. Our immunohistochemical studies provided hints of mitochondrial biogenesis and intracellular redistribution in GCs of growing follicles. A cellular model, human KGN cells (granulosa cell tumor cells, derived from growing follicles) was used to study aspects of mitochondrial dynamics. To elevate cAMP and thereby mimic gonadotropin actions, forskolin (FSK) was used, which increased KGN cell size and mitochondrial DNA within 24 h. MitoTracker experiments and ultrastructural 3D reconstruction revealed that FSK treatment induced the formation of elaborate mitochondrial networks. H89, a protein kinase A (PKA) inhibitor, reduced network formation. A proteomic analysis indicated that FSK among others elevated levels of regulators of the cytoskeleton and the steroidogenic enzyme CYP11A1, located in mitochondria, was more than 3-fold increased, implying that cAMP/PKA-associated structural changes go in parallel with the acquisition of steroidogenic competence of mitochondria in KGN cells. In summary, in situ observations showed increases in mitochondria and suggested intracellular trafficking in GCs during follicular growth and indicated that they may partially be under the control of gonadotropins/cAMP. In line with this, elevation of cAMP in KGN profoundly affected mitochondria dynamics in a PKA-dependent manner and implicated cytoskeletal changes.

2022-10-14 | PXD032160 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data